Streptomyces phage BeardedLady
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Detailed Information for Phage BeardedLady
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByMaggie Beard
Year Found2016
Location FoundFort Worth, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates32.954162 N, 97.285655 W Map
Discovery NotesThe soil was very soft and grainy. It had no clumps and was very loose. It was slightly damp with pieces of grass and root in it. It was dug out from 3-4 cm below the surface using a spoon and trough.
Naming NotesThe phage name BeardedLady is a play on my last name, which is Beard. Also, I am female, hence the name BeardedLady.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)49941
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCGGGCAGTGAT
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Other Cluster Members

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Aaronocolus (BD1)

Asten (BD1)

BarryBee (BD1)

BeardedLady (BD1)

Bovely (BD1)

Brataylor (BD1)

BryanRecycles (BD1)

Caliburn (BD1)

Celeste (BD1)

Chucky (BD1)

Danzina (BD1)

Dattran (BD1)

Dwayne (BD1)

Eddasa (BD1)

Ejemplo (BD1)

Emaanora (BD1)

EnochSoames (BD1)

Esperer (BD1)

Goby (BD1)

Godpower (BD1)

Hippo (BD1)

Hydra (BD1)

Indigo (BD1)

Izzy (BD1)

Jash (BD1)

Katalie (BD1)

Lannister (BD1)

Legacy (BD1)

Leviticus (BD1)

Lika (BD1)

Lorelei (BD1)

Maneekul (BD1)

Nabi (BD1)

Nanodon (BD1)

Nerdos (BD1)

Oliynyk (BD1)

Ozzie (BD1)

OzzyJ (BD1)

Phettuccine (BD1)

Rana (BD1)

RedBear (BD1)

Rusticus (BD1)

SarahRose (BD1)

Snorlax (BD1)

South40 (BD1)

Sujidade (BD1)

SunsetPointe (BD1)

TagePhighter (BD1)

Toma (BD1)

Triste (BD1)

TuanPN (BD1)

Unstoppable (BD1)

Werner (BD1)

Whatever (BD1)

Yasdnil (BD1)

Zemlya (BD1)

Alvy (BD2)

Amethyst (BD2)

Andris (BD2)

Animus (BD2)

BartholomewSD (BD2)

Bowden (BD2)

Caelum (BD2)

Daudau (BD2)

Diane (BD2)

ELB20 (BD2)

GirlDinner (BD2)

Haizum (BD2)

Hank144 (BD2)

Issmi (BD2)

Janus (BD2)

Jevington (BD2)

Loofah (BD2)

Marav (BD2)

Nishikigoi (BD2)

Omar (BD2)

Pablito (BD2)

Paedore (BD2)

Paolo (BD2)

Puginator (BD2)

R4 (BD2)

SqueakyClean (BD2)

Superstar (BD2)

Tefunt (BD2)

Thestral (BD2)

TinaBelcher (BD2)

Triumph (BD2)

TrvxScott (BD2)

Zainub (BD2)

Alsaber (BD3)

Amela (BD3)

Celery (BD3)

Conan (BD3)

Dexers (BD3)

ElGato (BD3)

phiCAM (BD3)

Provolone (BD3)

Saftant (BD3)

Vanseggelen (BD3)

Verabelle (BD3)

Verse (BD3)

Yosif (BD3)

BroPlease (BD4)

GreenWeasel (BD4)

phiHau3 (BD4)

StrepC (BD5)

Celia (BD6)

Itza (BD6)

Urza (BD6)

VieEnRose (BD6)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesMy plaques averaged between 1-2 mm in diameter. The plaques were circular, very clear, and formed halos. I had many individual plaques, but also some that would group together.
MorphotypeSiphoviridae
Number of Genes73
Has been Phamerated?Yes
Gene List

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BeardedLady_1

BeardedLady_2
(HNH endonuclease)

BeardedLady_3

BeardedLady_4

BeardedLady_5
(thioredoxin)

BeardedLady_6

BeardedLady_7
(terminase large subunit)

BeardedLady_8
(portal protein)

BeardedLady_9

BeardedLady_10
(scaffolding protein)

BeardedLady_11
(major capsid protein)

BeardedLady_12
(head-to-tail adaptor)

BeardedLady_13
(head-to-tail stopper)

BeardedLady_14

BeardedLady_15
(tail terminator)

BeardedLady_16

BeardedLady_17
(tail assembly chaperone)

BeardedLady_18
(tail assembly chaperone)

BeardedLady_19
(tape measure protein)

BeardedLady_20
(minor tail protein)

BeardedLady_21
(minor tail protein)

BeardedLady_22

BeardedLady_23

BeardedLady_24

BeardedLady_25

BeardedLady_26

BeardedLady_27
(endolysin)

BeardedLady_28

BeardedLady_29
(DNA binding protein)

BeardedLady_30
(immunity repressor)

BeardedLady_31

BeardedLady_32
(exonuclease)

BeardedLady_33

BeardedLady_34

BeardedLady_35
(deoxycytidylate deaminase)

BeardedLady_36
(DnaB-like dsDNA helicase)

BeardedLady_37
(holliday junction resolvase)

BeardedLady_38

BeardedLady_39
(DNA primase)

BeardedLady_40
(DNA primase)

BeardedLady_41

BeardedLady_42

BeardedLady_43

BeardedLady_44

BeardedLady_45
(DNA binding protein)

BeardedLady_46

BeardedLady_47
(ribonucleotide reductase)

BeardedLady_48
(Lsr2-like briding protein)

BeardedLady_49
(serine integrase)

BeardedLady_50
(ThyX-like thymidylate synthase)

BeardedLady_51

BeardedLady_52

BeardedLady_53
(DnaE-like DNA polymerase III alpha)

BeardedLady_54

BeardedLady_55

BeardedLady_56

BeardedLady_57
(deoxynucleoside monophosphate kinase)

BeardedLady_58

BeardedLady_59

BeardedLady_60

BeardedLady_61

BeardedLady_62

BeardedLady_63

BeardedLady_64

BeardedLady_65
(HNH endonuclease)

BeardedLady_66

BeardedLady_67
(OCR-like antirestriction protein)

BeardedLady_68

BeardedLady_69

BeardedLady_70

BeardedLady_71

BeardedLady_72

BeardedLady_73

BeardedLady_74
(acetyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF541403
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.0 x 10^9
Pitt Freezer Box# 36
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload