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Streptomyces phage VieEnRose
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Detailed Information for Phage VieEnRose
Discovery Information
Isolation HostStreptomyces sanglieri UNT16F27A
Found BySavannah Schultz-Loggie
Year Found2019
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.215092 N, 97.148592 W Map
Discovery NotesCollected Soil Sample underneath an unknown tree in the parking lot of CVS at 8:26pm. The soil was in a flower bed that had trees and an ivy like short bush plant that covered all the soil except for where the soil sample was taken. The soil was not very rich and moist, but it was still a dark color. It is very fine soil, and there were pieces of trash (Cheetos bag and other unidentifiable wrappers) near the collection site. Dug down an inch with a clean spoon and then collected the soil using the spoon to put about 5mL in a 14mL test tube. Outside temperature was 88°F or 31.1°C. It was clear skies and dark outside, and the sun had just set and it was not windy. GPS coordinates for the sample are N 33° 12' 54.331", W 97° 8' 54.931".
Naming NotesI named my phage after one of my favorite songs. The name of the sone is La Vie En Rose by Édith Piaf.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 26, 2021
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage94
Genome length (bp)50193
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCTGCGTCAT
GC Content68.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD6
Cluster Life CycleTemperate
Lysogeny NotesNegative for lysogeny test
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 1/26/2022)
Plaque NotesIt's circular and clear and has a diameter of .8 cm.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.4x10^8 pfu/mL
Pitt Freezer Box# 104
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload