Streptomyces phage VieEnRose
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Detailed Information for Phage VieEnRose
Discovery Information
Isolation HostStreptomyces sanglieri UNT16F27A
Found BySavannah Schultz-Loggie
Year Found2019
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.215092 N, 97.148592 W Map
Discovery NotesCollected Soil Sample underneath an unknown tree in the parking lot of CVS at 8:26pm. The soil was in a flower bed that had trees and an ivy like short bush plant that covered all the soil except for where the soil sample was taken. The soil was not very rich and moist, but it was still a dark color. It is very fine soil, and there were pieces of trash (Cheetos bag and other unidentifiable wrappers) near the collection site. Dug down an inch with a clean spoon and then collected the soil using the spoon to put about 5mL in a 14mL test tube. Outside temperature was 88°F or 31.1°C. It was clear skies and dark outside, and the sun had just set and it was not windy. GPS coordinates for the sample are N 33° 12' 54.331", W 97° 8' 54.931".
Naming NotesI named my phage after one of my favorite songs. The name of the sone is La Vie En Rose by Édith Piaf.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 26, 2021
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage94
Genome length (bp)50193
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCTGCGTCAT
GC Content68.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD6
Cluster Life CycleTemperate
Lysogeny NotesNegative for lysogeny test
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesIt's circular and clear and has a diameter of .8 cm.
Has been Phamerated?Yes
Gene List

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VieEnRose_1

VieEnRose_2
(HNH endonuclease)

VieEnRose_4
(phosphoesterase)

VieEnRose_5
(terminase, small subunit)

VieEnRose_6
(terminase, large subunit)

VieEnRose_7
(portal protein)

VieEnRose_8

VieEnRose_9
(scaffolding protein)

VieEnRose_10
(major capsid protein)

VieEnRose_11
(head-to-tail adaptor)

VieEnRose_12
(head-to-tail stopper)

VieEnRose_13

VieEnRose_14
(tail terminator)

VieEnRose_15
(major tail protein)

VieEnRose_16
(tail assembly chaperone)

VieEnRose_17
(tail assembly chaperone)

VieEnRose_18
(tape measure protein)

VieEnRose_19
(minor tail protein)

VieEnRose_20
(minor tail protein)

VieEnRose_21

VieEnRose_22

VieEnRose_23

VieEnRose_24

VieEnRose_25

VieEnRose_26
(minor tail protein)

VieEnRose_27

VieEnRose_28
(endolysin)

VieEnRose_29

VieEnRose_30
(DNA binding protein)

VieEnRose_31
(immunity repressor)

VieEnRose_32

VieEnRose_33
(exonuclease)

VieEnRose_34

VieEnRose_35

VieEnRose_36
(DnaB-like dsDNA helicase)

VieEnRose_37
(Holliday junction resolvase)

VieEnRose_38

VieEnRose_39
(DNA primase)

VieEnRose_40
(DNA primase)

VieEnRose_41

VieEnRose_42

VieEnRose_43

VieEnRose_44

VieEnRose_45
(exonuclease)

VieEnRose_46

VieEnRose_47
(DNA binding protein)

VieEnRose_48
(Lsr2-like DNA bridging protein)

VieEnRose_49

VieEnRose_50
(serine integrase)

VieEnRose_51

VieEnRose_52

VieEnRose_53

VieEnRose_54
(DnaE-like DNA polymerase III (alpha))

VieEnRose_55

VieEnRose_56

VieEnRose_57

VieEnRose_58

VieEnRose_59

VieEnRose_60

VieEnRose_61
(deoxynucleoside monophosphate kinase)

VieEnRose_62

VieEnRose_63

VieEnRose_64

VieEnRose_65

VieEnRose_66

VieEnRose_67

VieEnRose_68

VieEnRose_69

VieEnRose_70

VieEnRose_71

VieEnRose_72

VieEnRose_73

VieEnRose_74
(DNA methylase)

VieEnRose_75

VieEnRose_76

VieEnRose_77

VieEnRose_78

VieEnRose_79
(acetyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON526983
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.4x10^8 pfu/mL
Pitt Freezer Box# 104
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload