Streptomyces phage Unstoppable
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Detailed Information for Phage Unstoppable
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByJasmine Hatter
Year Found2020
Location FoundDenton, TX United States of America
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.212569 N, 97.14687 W Map
Discovery NotesCollected soil in Denton, Texas. The temperature was about 100 degrees F and it was muddy from the sprinklers from earlier in the morning. The location is under a tree near al dorm. I dug about the top 1 inch of soil from between the tree roots and placed in a plastic bag.
Naming NotesMy Phage can not be beat, so I thought it would great to name it "unstoppable"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1778
Genome length (bp)49556
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGCAGTGAT
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Other Cluster Members

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Aaronocolus (BD1)

Asten (BD1)

BarryBee (BD1)

BeardedLady (BD1)

Bovely (BD1)

Brataylor (BD1)

BryanRecycles (BD1)

Caliburn (BD1)

Celeste (BD1)

Chucky (BD1)

Danzina (BD1)

Dattran (BD1)

Dwayne (BD1)

Eddasa (BD1)

Ejemplo (BD1)

Emaanora (BD1)

EnochSoames (BD1)

Esperer (BD1)

Goby (BD1)

Godpower (BD1)

Hippo (BD1)

Hydra (BD1)

Indigo (BD1)

Izzy (BD1)

Jash (BD1)

Katalie (BD1)

Lannister (BD1)

Legacy (BD1)

Leviticus (BD1)

Lika (BD1)

Lorelei (BD1)

Maneekul (BD1)

Nabi (BD1)

Nanodon (BD1)

Nerdos (BD1)

Oliynyk (BD1)

Ozzie (BD1)

OzzyJ (BD1)

Phettuccine (BD1)

Rana (BD1)

RedBear (BD1)

Rusticus (BD1)

SarahRose (BD1)

Snorlax (BD1)

South40 (BD1)

Sujidade (BD1)

SunsetPointe (BD1)

TagePhighter (BD1)

Toma (BD1)

Triste (BD1)

TuanPN (BD1)

Unstoppable (BD1)

Werner (BD1)

Whatever (BD1)

Yasdnil (BD1)

Zemlya (BD1)

Alvy (BD2)

Amethyst (BD2)

Andris (BD2)

Animus (BD2)

BartholomewSD (BD2)

Bowden (BD2)

Caelum (BD2)

Daudau (BD2)

Diane (BD2)

ELB20 (BD2)

GirlDinner (BD2)

Haizum (BD2)

Hank144 (BD2)

Issmi (BD2)

Janus (BD2)

Jevington (BD2)

Loofah (BD2)

Marav (BD2)

Nishikigoi (BD2)

Omar (BD2)

Pablito (BD2)

Paedore (BD2)

Paolo (BD2)

Puginator (BD2)

R4 (BD2)

SqueakyClean (BD2)

Superstar (BD2)

Tefunt (BD2)

Thestral (BD2)

TinaBelcher (BD2)

Triumph (BD2)

TrvxScott (BD2)

Zainub (BD2)

Alsaber (BD3)

Amela (BD3)

Celery (BD3)

Conan (BD3)

Dexers (BD3)

ElGato (BD3)

Kaine (BD3)

Pavo (BD3)

phiCAM (BD3)

Provolone (BD3)

Saftant (BD3)

Speedwell (BD3)

Vanseggelen (BD3)

Verabelle (BD3)

Verse (BD3)

Yosif (BD3)

BroPlease (BD4)

GreenWeasel (BD4)

phiHau3 (BD4)

StrepC (BD5)

Celia (BD6)

Itza (BD6)

Urza (BD6)

VieEnRose (BD6)

Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesThe phage are clear and circular with an average diameter of 2 mm.
Has been Phamerated?Yes
Gene List

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Unstoppable_1

Unstoppable_2
(HNH endonuclease)

Unstoppable_3

Unstoppable_4
(thioredoxin)

Unstoppable_5

Unstoppable_6
(terminase large subunit)

Unstoppable_7
(portal protein)

Unstoppable_8

Unstoppable_9
(scaffolding protein)

Unstoppable_10
(major capsid protein)

Unstoppable_11
(head-to-tail adaptor)

Unstoppable_12
(head-to-tail stopper)

Unstoppable_13

Unstoppable_14
(tail terminator)

Unstoppable_15

Unstoppable_16
(tail assembly chaperone)

Unstoppable_17
(tail assembly chaperone)

Unstoppable_18
(tape measure protein)

Unstoppable_19
(minor tail protein)

Unstoppable_20
(minor tail protein)

Unstoppable_21

Unstoppable_22

Unstoppable_23

Unstoppable_24

Unstoppable_25

Unstoppable_26
(endolysin)

Unstoppable_27

Unstoppable_28

Unstoppable_29
(immunity repressor)

Unstoppable_30

Unstoppable_31
(exonuclease)

Unstoppable_32

Unstoppable_33

Unstoppable_34
(deoxycytidylate deaminase)

Unstoppable_35
(DnaB-like dsDNA helicase)

Unstoppable_36
(holliday junction resolvase)

Unstoppable_37

Unstoppable_38
(DNA primase)

Unstoppable_39
(DNA primase)

Unstoppable_40

Unstoppable_41

Unstoppable_42

Unstoppable_43
(MRE11 double-strand break endo/exonuclease)

Unstoppable_44

Unstoppable_45
(DNA binding protein)

Unstoppable_46

Unstoppable_47
(ribonucleotide reductase)

Unstoppable_48
(Lsr2-like DNA bridging protein)

Unstoppable_49
(serine integrase)

Unstoppable_50
(ThyX-like thymidylate synthase)

Unstoppable_51

Unstoppable_52

Unstoppable_53
(DnaE-like DNA polymerase III alpha)

Unstoppable_54

Unstoppable_55

Unstoppable_56

Unstoppable_57
(deoxynucleoside monophosphate kinase)

Unstoppable_58

Unstoppable_59

Unstoppable_60

Unstoppable_61

Unstoppable_62

Unstoppable_63

Unstoppable_64
(HNH endonuclease)

Unstoppable_65

Unstoppable_66

Unstoppable_67

Unstoppable_68

Unstoppable_69

Unstoppable_70

Unstoppable_71

Unstoppable_72

Unstoppable_73
(acetyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOL455894
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8*10^9 pfu/mL
Pitt Freezer Box# 118
Pitt Freezer Box Grid# B5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload