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Streptomyces phage Animus
Animus EM Picture
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Detailed Information for Phage Animus
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Former namesTDDu
Found ByTony Du
Year Found2018
Location FoundCarrollton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.001746 N, 96.864174 W Map
Discovery NotesFound on 01/28/2018 at 12:00pm on a sunny day where the weather was at 21℃. The soil was collected right by an elementary school underneath a large tree. The soil itself was very crumbly and dry, with no signs of moisture at the time of collection. The color was extremely dark brown, verging on black.
Naming Notes"Animus" is a Latin word that is translated to mean the soul, spirit, and the mind. I had wanted to name it after such a word because of the same things we instill into these phages upon its discovery. I had given a soul, per say, to an organism that had previously been unknown to the entire world, giving it a name to embody the very sensation many other students have when discovering their own bacteriophages.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage320
Genome length (bp)50793
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCGTGTCTT
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Lysogeny NotesRegarding lysogeny, because a spot was seen on the ten to the zero spot and no spots were seen on any of the dilution spots, this showed evidence that Animus’ phage particles overwhelmed the host bacteria, causing it to die, not because the phage infected the host. Because of this, it could mean Animus could produce a lysogen, where it would go through the lysogenic life cycle. This brings up the possibility that Animus would be classified as a temperate phage but regardless, we cannot definitively say that Animus is able to actually go through the lysogenic life cycle— further testing would need to be done.
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesEach of the plaques ranged anywhere between 0.5 mm and 1 mm in diameter and were all comet shaped.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN234192
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 75
Pitt Freezer Box Grid# A6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload