Streptomyces phage Legacy
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Detailed Information for Phage Legacy
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByJermiah Crowder
Year Found2020
Location FoundThe Colony, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.0486 N, 96.9191 W Map
Discovery NotesUsing Protocol 5.1 Collecting Environmental Samples, soil was collected from The Colony, Texas (33.0486°N, 96.9191°W) in order to isolate a bacteriophage. The air temperature surrounding the soil at time 17:28 when soil was retrieved was 93°F. The soil appeared a little moist around below the surface while at the surface, it was dry and flaky (Figure 0). Many organisms such as worms and other insects were found near the soil collection site. I dug 4 cm below the surface to collect the soil and placed it with a clean spoon into a plastic sandwich bag. The sample was named "Colony" soil.
Naming NotesI chose Legacy because I have been obsessed with the name since I was in middle school. I plan to name my first born daughter Legacy and I want her to know that she was named after the phage I discovered.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage122
Genome length (bp)49941
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGCAGTGAT
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionCabrini University
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesThe phage is clear and circular with an average diameter of 1 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.4x10^9 pfu/mL
Pitt Freezer Box# 117
Pitt Freezer Box Grid# H1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload