The Actinobacteriophage Database

Streptomyces phage Triste

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(S. griseus NBRC13350)

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(S. scabiei 82-22)

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Detailed Information for Phage Triste
Discovery Information
Isolation Host	Streptomyces lividans JI 1326
Found By	Caroline Chou and Hannah Mitchell
Year Found	2019
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	39°C
GPS Coordinates	38.636389 N, 90.293611 W Map
Discovery Notes	In Forest Park, next to a big tree, lots of undergrowth, insects, decomposing leaves, plant roots around, dug 10cm in to the soil, soil crumbly but moist.
Naming Notes	Pronounced "Treest" Triste means sad in French.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Nov 20, 2020
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	101
Genome length (bp)	51568
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGGCCAGTGAT
GC Content	65.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BD
Subcluster	BD1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Aaronocolus (BD1) Asten (BD1) BarryBee (BD1) BeardedLady (BD1) Bovely (BD1) Brataylor (BD1) BryanRecycles (BD1) Caliburn (BD1) Celeste (BD1) Chucky (BD1) Danzina (BD1) Dattran (BD1) Dwayne (BD1) Eddasa (BD1) Ejemplo (BD1) Emaanora (BD1) EnochSoames (BD1) Esperer (BD1) Goby (BD1) Godpower (BD1) Hippo (BD1) Hydra (BD1) Indigo (BD1) Izzy (BD1) Jash (BD1) Katalie (BD1) Lannister (BD1) Legacy (BD1) Leviticus (BD1) Lika (BD1) Lorelei (BD1) Maneekul (BD1) Nabi (BD1) Nanodon (BD1) Nerdos (BD1) Oliynyk (BD1) Ozzie (BD1) OzzyJ (BD1) Phettuccine (BD1) Rana (BD1) RedBear (BD1) Rusticus (BD1) SarahRose (BD1) Snorlax (BD1) South40 (BD1) Sujidade (BD1) SunsetPointe (BD1) TagePhighter (BD1) Toma (BD1) Triste (BD1) TuanPN (BD1) Unstoppable (BD1) Werner (BD1) Whatever (BD1) Yasdnil (BD1) Zemlya (BD1) Alvy (BD2) Amethyst (BD2) Andris (BD2) Animus (BD2) BartholomewSD (BD2) Bowden (BD2) Caelum (BD2) Daudau (BD2) Diane (BD2) ELB20 (BD2) GirlDinner (BD2) Haizum (BD2) Hank144 (BD2) Issmi (BD2) Janus (BD2) Jevington (BD2) Loofah (BD2) Marav (BD2) Nishikigoi (BD2) Omar (BD2) Pablito (BD2) Paedore (BD2) Paolo (BD2) Puginator (BD2) R4 (BD2) SqueakyClean (BD2) Superstar (BD2) Tefunt (BD2) Thestral (BD2) TinaBelcher (BD2) Triumph (BD2) TrvxScott (BD2) Zainub (BD2) Alsaber (BD3) Amela (BD3) Celery (BD3) Conan (BD3) Dexers (BD3) ElGato (BD3) phiCAM (BD3) Provolone (BD3) Saftant (BD3) Vanseggelen (BD3) Verabelle (BD3) Verse (BD3) Yosif (BD3) BroPlease (BD4) GreenWeasel (BD4) phiHau3 (BD4) StrepC (BD5) Celia (BD6) Itza (BD6) Urza (BD6) VieEnRose (BD6)
Annotating Institution	Washington University in St. Louis
Annotation Status	In GenBank
Plaque Notes	around 3mm in diameter, sometimes had red ring of bacterial contamination outlining it, slightly turbid (became less turbid as more rounds of purification were done)
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Triste_1 Triste_2 (HNH endonuclease) Triste_3 Triste_4 (thioredoxin) Triste_5 (terminase small subunit) Triste_6 (terminase large subunit) Triste_7 (portal protein) Triste_8 (capsid maturation protease and MuF-like fusion protein) Triste_9 (scaffolding protein) Triste_10 (major capsid protein) Triste_11 (head-to-tail adaptor) Triste_12 (head-to-tail stopper) Triste_13 Triste_14 (tail terminator) Triste_15 (major tail protein) Triste_16 (tail assembly chaperone) Triste_17 (tail assembly chaperone) Triste_18 (tape measure protein) Triste_19 (minor tail protein) Triste_20 (minor tail protein) Triste_21 Triste_22 (minor tail protein) Triste_23 (minor tail protein) Triste_24 Triste_25 (endolysin) Triste_26 Triste_27 Triste_28 (immunity repressor) Triste_29 Triste_30 (exonuclease) Triste_31 Triste_32 Triste_33 Triste_34 (deoxycytidylate deaminase) Triste_35 (DnaB-like dsDNA helicase) Triste_36 (holliday junction resolvase) Triste_37 Triste_38 (DNA primase) Triste_39 (DNA primase) Triste_40 Triste_41 Triste_42 (helix-turn-helix DNA binding domain protein) Triste_43 (MRE11 double-strand break endo/exonuclease) Triste_44 Triste_45 (helix-turn-helix DNA binding domain protein) Triste_46 Triste_47 Triste_48 Triste_49 (DNA methylase) Triste_50 Triste_51 (serine integrase) Triste_52 (ThyX-like thymidylate synthase) Triste_53 Triste_54 Triste_55 (DnaE-like DNA polymerase III) Triste_56 Triste_57 Triste_58 Triste_59 (deoxynucleoside monophosphate kinase) Triste_60 Triste_61 Triste_62 Triste_63 Triste_64 Triste_65 Triste_66 (HNH endonuclease) Triste_67 Triste_68 (OCR-like antirestriction protein) Triste_69 Triste_70 Triste_71 Triste_72 Triste_73 Triste_74 (acetyltransferase)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MZ648040
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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