Streptomyces phage Bowden
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Detailed Information for Phage Bowden
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByStormie Bowden
Year Found2016
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.210659 N, 97.147261 W Map
Discovery NotesI took the soil sample at 9:30am on September 12, 2016. I dug approximately half an inch into the ground. It was 61 degrees Fahrenheit, and the soil was slightly moist.
Naming NotesBecause I did the research to find the phage, I named it after myself.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 7, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage156
Genome length (bp)52603
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content67.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesThe plaques were clear, circular, and measured 1mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Bowden_1

Bowden_2
(HNH endonuclease)

Bowden_4

Bowden_5

Bowden_6
(terminase small subunit)

Bowden_7
(terminase large subunit)

Bowden_8
(portal protein)

Bowden_9
(capsid maturation protease)

Bowden_10
(scaffolding protein)

Bowden_11
(major capsid protein)

Bowden_12
(head-to-tail connector complex protein)

Bowden_13
(head-to-tail connector complex protein)

Bowden_14

Bowden_15

Bowden_16
(major tail protein)

Bowden_17
(tail assembly chaperone)

Bowden_18
(tail assembly chaperone)

Bowden_19
(tape measure protein)

Bowden_20
(minor tail protein)

Bowden_21
(minor tail protein)

Bowden_22

Bowden_23

Bowden_24

Bowden_25
(minor tail protein)

Bowden_26

Bowden_27
(lysin A)

Bowden_28

Bowden_29
(immunity repressor)

Bowden_30

Bowden_31
(exonuclease)

Bowden_32

Bowden_33

Bowden_34

Bowden_35
(deoxycytidylate deaminase)

Bowden_36
(DnaB-like dsDNA helicase)

Bowden_37
(holliday junction resolvase)

Bowden_38

Bowden_39
(DNA primase)

Bowden_40
(DNA primase)

Bowden_41

Bowden_42

Bowden_43
(endonuclease)

Bowden_44

Bowden_45
(helix-turn-helix DNA binding domain protein)

Bowden_46

Bowden_47
(ribonucleotide reductase)

Bowden_48
(methyltransferase)

Bowden_49

Bowden_50

Bowden_51

Bowden_52
(helix-turn-helix DNA binding domain protein)

Bowden_53
(integrase)

Bowden_54

Bowden_55
(ThyX-like thymidylate synthase)

Bowden_56

Bowden_57

Bowden_58

Bowden_59

Bowden_60

Bowden_61

Bowden_62
(deoxynucleoside monophosphate kinase)

Bowden_63

Bowden_64

Bowden_65

Bowden_66

Bowden_67

Bowden_68

Bowden_69

Bowden_70

Bowden_71

Bowden_72

Bowden_73

Bowden_74

Bowden_75

Bowden_76

Bowden_77

Bowden_78

Bowden_79
(acetyltransferase)

Bowden_80

Bowden_81

Bowden_82

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK392363
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^8 pfu/mL
Date of SEA Lysate Titering Nov 7, 2016
Pitt Freezer Box# 36
Pitt Freezer Box Grid# D7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload