Discovery Information |
Isolation Host | Streptomyces griseus ATCC 10137 |
Found By | Terry Lee, Chere Petty, Steven Caruso |
Year Found | 2015 |
Location Found | Kilauea, HI USA |
Finding Institution | University of Maryland, Baltimore County |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 22.174618 N, 159.435647 W Map |
Discovery Notes | Sample was collected by Dr. Terry Lee in Kilauea from volcanic soil and provided to Chere Petty, then enriched on Streptomyces griseus griseus ATCC 10137 by SMC. |
Naming Notes | Nano- for tiny, -odon for tooth = tiny tooth. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 5, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1201 |
Genome length (bp) | 50082 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGGGCAGTGAT |
GC Content | 65.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BD |
Subcluster | BD1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Clear plaques 0.5-2.5 mm in d. with rough edges after 48 hrs at 30 C. Phage dim are: capsid l = 48.2 nm, w = 45.6 nm, tail l = 151.3 nm, n = 2. |
Morphotype | Siphoviridae |
Number of Genes | 75 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KX344445 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2015UMBCNanodonSMC |
SEA Lysate Titer |
1.3 x 10^9 pfu/ml |
Date of SEA Lysate Titering |
Nov 18, 2015 |
Pitt Freezer Box# |
19 |
Pitt Freezer Box Grid# |
F1 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
Virtual Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |