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Streptomyces phage Nishikigoi
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Detailed Information for Phage Nishikigoi
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByKhoi Tran
Year Found2016
Location FoundCarrollton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates32.99963 N, 96.894871 W Map
Discovery NotesCollected from my friend's backyard.
My friend is also my lab partner.
Soil was extracted on September 12, 2016.
Temperature at the time was 20 degrees Celsius
Extracted at 7:14 AM

The site of extraction was next to a concrete slab which is under a palvika plant. The site is also where grease and oil cooking oil would be deposited. The site is a shaded area and was able to stay moist.

The soil extracted was clumped into 3 clumps. It was a dark color and was damp.
Naming NotesNishikigoi is how carp or koi fish is said in Japanese. This name is homage to my own name as well as my significant other's ethnic background.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 23, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage864
Genome length (bp)50660
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Lysogeny NotesBased on tests, Nishikigoi appears to be lysogenic
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesRound, circular plaques; approximately 2 mm in diameter
Has been Phamerated?Yes
Gene List

Click to View

Nishikigoi_1

Nishikigoi_2
(HNH endonuclease)

Nishikigoi_3

Nishikigoi_4
(thioredoxin)

Nishikigoi_5
(terminase small subunit)

Nishikigoi_6
(terminase large subunit)

Nishikigoi_7
(portal protein)

Nishikigoi_8
(capsid maturation protease)

Nishikigoi_9
(scaffolding protein)

Nishikigoi_10
(major capsid protein)

Nishikigoi_11
(head-to-tail connector complex protein)

Nishikigoi_12
(head-to-tail connector complex protein)

Nishikigoi_13

Nishikigoi_14

Nishikigoi_15
(major tail protein)

Nishikigoi_16
(tail assembly chaperone)

Nishikigoi_17
(tail assembly chaperone)

Nishikigoi_18
(tape measure protein)

Nishikigoi_19
(minor tail protein)

Nishikigoi_20
(minor tail protein)

Nishikigoi_21

Nishikigoi_22

Nishikigoi_23

Nishikigoi_24
(minor tail protein)

Nishikigoi_25

Nishikigoi_26
(lysin A)

Nishikigoi_27

Nishikigoi_28

Nishikigoi_29
(immunity repressor)

Nishikigoi_30

Nishikigoi_31
(exonuclease)

Nishikigoi_32

Nishikigoi_33

Nishikigoi_34

Nishikigoi_35

Nishikigoi_36
(deoxycytidylate deaminase)

Nishikigoi_37
(DnaB-like dsDNA helicase)

Nishikigoi_38
(holliday junction resolvase)

Nishikigoi_39

Nishikigoi_40
(DNA primase)

Nishikigoi_41
(DNA primase)

Nishikigoi_42

Nishikigoi_43

Nishikigoi_44

Nishikigoi_45
(endonuclease)

Nishikigoi_46

Nishikigoi_47
(helix-turn-helix DNA binding domain protein)

Nishikigoi_48

Nishikigoi_49
(ribonucleotide reductase)

Nishikigoi_50

Nishikigoi_51

Nishikigoi_52
(helix-turn-helix DNA binding domain protein)

Nishikigoi_53
(integrase)

Nishikigoi_54
(ThyX-like thymidylate synthase)

Nishikigoi_55

Nishikigoi_56

Nishikigoi_57

Nishikigoi_58

Nishikigoi_59

Nishikigoi_60

Nishikigoi_61

Nishikigoi_62

Nishikigoi_63
(deoxynucleoside monophosphate kinase)

Nishikigoi_64

Nishikigoi_65

Nishikigoi_66

Nishikigoi_67

Nishikigoi_68

Nishikigoi_69

Nishikigoi_70

Nishikigoi_71

Nishikigoi_72

Nishikigoi_73

Nishikigoi_74

Nishikigoi_75

Nishikigoi_76

Nishikigoi_77

Nishikigoi_78
(acetyltransferase)

Nishikigoi_79

Nishikigoi_80

Nishikigoi_81

Nishikigoi_82

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK392364
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^8 pfu/mL
Pitt Freezer Box# 36
Pitt Freezer Box Grid# G1
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload