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Streptomyces phage Amethyst

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(S. griseus NBRC13350)

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(S. scabiei 82-22)

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Detailed Information for Phage Amethyst
Discovery Information
Isolation Host	Streptomyces xanthochromogenes NRRL B-5410
Found By	Elizabeth Spangler
Year Found	2016
Location Found	Denton, TX USA
Finding Institution	University of North Texas
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	33.2125 N, 97.14722 W Map
Discovery Notes	Soil found in Denton, TX; Temperature was 85° F at soil sample collection. The date and time of soil sample collection was 9/9/16 at 11:08am; the soil collected was dry, loose soil. The MTL of this phage is 1.2x10^8 pfu/mL; the average diameter of plaques is 2mm; the DNA concentration is 131.8 and the DNA/RNA ratio is 1.93. In a host range test with the bacteria S. venezuelae, S. albus, S. virginiae, S. griseus, and S. azureus, no plaques were formed using Amethyst.
Naming Notes	The name for this phage came from the February birthstone, Amethyst.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 30, 2017
Sequencing Facility	University of North Texas
Shotgun Sequencing Method	Illumina Sequencing
Genome length (bp)	49372
Character of genome ends	Unknown
Overhang Length	11 bases
Overhang Sequence	CGCCACGTCTT
GC Content	67.2%
Sequencing Notes	at position 19553 approximately 33% of reads contain a G instead of consensus A
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BD
Subcluster	BD2
Cluster Life Cycle	Temperate
Lysogeny Notes	Titer tests concluded that Amethyst isn't a lysogen.
Other Cluster Members	Click to View Aaronocolus (BD1) Asten (BD1) BarryBee (BD1) BeardedLady (BD1) Bovely (BD1) Brataylor (BD1) BryanRecycles (BD1) Caliburn (BD1) Celeste (BD1) Chucky (BD1) Danzina (BD1) Dattran (BD1) Dwayne (BD1) Eddasa (BD1) Ejemplo (BD1) EnochSoames (BD1) Esperer (BD1) Goby (BD1) Godpower (BD1) Hippo (BD1) Hydra (BD1) Indigo (BD1) Izzy (BD1) Jash (BD1) Katalie (BD1) Lannister (BD1) Legacy (BD1) Leviticus (BD1) Lika (BD1) Lorelei (BD1) Maneekul (BD1) Nabi (BD1) Nanodon (BD1) Nerdos (BD1) Oliynyk (BD1) Ozzie (BD1) OzzyJ (BD1) Phettuccine (BD1) Rana (BD1) RedBear (BD1) Rusticus (BD1) SarahRose (BD1) Snorlax (BD1) South40 (BD1) Sujidade (BD1) SunsetPointe (BD1) TagePhighter (BD1) Toma (BD1) Triste (BD1) TuanPN (BD1) Unstoppable (BD1) Werner (BD1) Whatever (BD1) Yasdnil (BD1) Zemlya (BD1) Alvy (BD2) Amethyst (BD2) Andris (BD2) Animus (BD2) BartholomewSD (BD2) Bowden (BD2) Caelum (BD2) Daudau (BD2) Diane (BD2) ELB20 (BD2) Haizum (BD2) Hank144 (BD2) Issmi (BD2) Janus (BD2) Marav (BD2) Nishikigoi (BD2) Omar (BD2) Pablito (BD2) Paedore (BD2) R4 (BD2) SqueakyClean (BD2) Tefunt (BD2) Thestral (BD2) TinaBelcher (BD2) Triumph (BD2) TrvxScott (BD2) Alsaber (BD3) Amela (BD3) Celery (BD3) Dexers (BD3) phiCAM (BD3) Provolone (BD3) Saftant (BD3) Vanseggelen (BD3) Verabelle (BD3) Verse (BD3) Yosif (BD3) BroPlease (BD4) GreenWeasel (BD4) phiHau3 (BD4) StrepC (BD5) Celia (BD6) Itza (BD6) Urza (BD6) VieEnRose (BD6)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	79
Has been Phamerated?	Yes
Gene List	Click to View Amethyst_1 Amethyst_2 (HNH endonuclease) Amethyst_3 Amethyst_4 (thioredoxin) Amethyst_5 Amethyst_6 (terminase large subunit) Amethyst_7 (portal protein) Amethyst_8 Amethyst_9 (scaffolding protein) Amethyst_10 (major capsid protein) Amethyst_11 (head-to-tail adaptor) Amethyst_12 (head-to-tail stopper) Amethyst_13 Amethyst_14 (tail terminator) Amethyst_15 (major tail protein) Amethyst_16 (tail assembly chaperone) Amethyst_17 (tail assembly chaperone) Amethyst_18 (tape measure protein) Amethyst_19 (minor tail protein) Amethyst_20 (minor tail protein) Amethyst_21 Amethyst_22 (minor tail protein) Amethyst_23 Amethyst_24 (minor tail protein) Amethyst_25 Amethyst_26 (endolysin) Amethyst_27 Amethyst_28 (DNA binding protein) Amethyst_29 (immunity repressor) Amethyst_30 Amethyst_31 (exonuclease) Amethyst_32 Amethyst_33 Amethyst_34 (deoxycytidylate deaminase) Amethyst_35 (DnaB-like dsDNA helicase) Amethyst_36 (holliday junction resolvase) Amethyst_37 Amethyst_38 (DNA primase) Amethyst_39 (DNA primase) Amethyst_40 Amethyst_41 Amethyst_42 Amethyst_43 (MRE11 double-strand break endo/exonuclease) Amethyst_44 Amethyst_45 (DNA binding protein) Amethyst_46 Amethyst_47 (ribonucleotide reductase) Amethyst_48 Amethyst_49 Amethyst_50 (DNA binding protein) Amethyst_51 (serine integrase) Amethyst_52 Amethyst_53 (ThyX-like thymidylate synthase) Amethyst_54 Amethyst_55 Amethyst_56 Amethyst_57 Amethyst_58 Amethyst_59 Amethyst_60 Amethyst_61 Amethyst_62 (deoxynucleoside monophosphate) Amethyst_63 Amethyst_64 Amethyst_65 Amethyst_66 Amethyst_67 Amethyst_68 (OCR-like antirestriction protein) Amethyst_69 Amethyst_70 Amethyst_71 Amethyst_72 Amethyst_73 Amethyst_74 Amethyst_75 Amethyst_76 (acetyltransferase) Amethyst_77 Amethyst_78 Amethyst_79
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF766044
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	6.0 x 10^8
Pitt Freezer Box#	36
Pitt Freezer Box Grid#	C9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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