Detailed Information for Phage BeardedLady |
Discovery Information |
Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
Found By | Maggie Beard |
Year Found | 2016 |
Location Found | Fort Worth, TX USA |
Finding Institution | University of North Texas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Unknown |
Isolation Temperature | 30°C |
GPS Coordinates | 32.954162 N, 97.285655 W Map |
Discovery Notes | The soil was very soft and grainy. It had no clumps and was very loose. It was slightly damp with pieces of grass and root in it. It was dug out from 3-4 cm below the surface using a spoon and trough. |
Naming Notes | The phage name BeardedLady is a play on my last name, which is Beard. Also, I am female, hence the name BeardedLady. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 14, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Genome length (bp) | 49941 |
Character of genome ends | Unknown |
Overhang Length | 11 bases |
Overhang Sequence | CGGGCAGTGAT |
GC Content | 66.2% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BD |
Subcluster | BD1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | My plaques averaged between 1-2 mm in diameter. The plaques were circular, very clear, and formed halos. I had many individual plaques, but also some that would group together. |
Morphotype | Siphoviridae |
Number of Genes | 74 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MF541403 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
6.0 x 10^9 |
Pitt Freezer Box# |
36 |
Pitt Freezer Box Grid# |
D3 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |