| Detailed Information for Phage Beaver |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Carlee Hutchins, Griffin Hopkins, Tiffany Knowles |
| Year Found | 2018 |
| Location Found | Arkadelphia, AR USA |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.1762 N, 93.0983 W Map |
| Discovery Notes | Discovered near local dam outpour on rocks near the water (5ft from water flow) on sunny/partly cloudy, 92*F day |
| Naming Notes | We chose the name Beaver because Beavers build dams and we found our phage near a dam. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 29, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 138 |
| Genome length (bp) | 77394 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 58.9% |
| Sequencing Notes | This genome was sequenced as part of a deliberately mixed sample (with SheckWes), and determined to belong to phage Beaver based on restriction digest patterns. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | In GenBank |
| Plaque Notes | 1mm diameter plaques, typically clean entire plates until the -3 dilution in serial dilutions |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK967390 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.26x10^9 pfu/mL |
| Pitt Freezer Box# |
78 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
