Gordonia phage Chelms
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Detailed Information for Phage Chelms
Discovery Information
Isolation HostGordonia terrae 3612
Found ByCassie Helmer
Year Found2016
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.4445 N, 79.9615 W Map
Discovery NotesThe soil sample was collected from the grass area outside Braierl Recreation Center in the Peterson Event Center. The coordinates for where the soil was collected are 40.4445 N and 79.9615 W. The sample was taken from the edge of where the grass was growing next to a large block of stone. The soil was dry and had roots running through it. It was taken from the top layer of dirt. The soil was collected at 3;30 PM. The temperature was 75ºF and it was in the bag for about a day before being added to phage buffer.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2016
Sequencing FacilityUniversity of Pittsburgh Genomics and Proteomics Core Laboratories
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1063
Genome length (bp)76261
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 12/15/2018)
Plaque NotesChelms produces small plaques. Some give a clear appearance and others give a haloed appearance.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3x10^10 pfu/mL
Date of SEA Lysate Titering Oct 26, 2016
Pitt Freezer Box# 42
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload