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Gordonia phage Chelms
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Detailed Information for Phage Chelms
Discovery Information
Isolation HostGordonia terrae 3612
Found ByCassie Helmer
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.4445 N, 79.9615 W Map
Discovery NotesThe soil sample was collected from the grass area outside Braierl Recreation Center in the Peterson Event Center. The coordinates for where the soil was collected are 40.4445 N and 79.9615 W. The sample was taken from the edge of where the grass was growing next to a large block of stone. The soil was dry and had roots running through it. It was taken from the top layer of dirt. The soil was collected at 3;30 PM. The temperature was 75ºF and it was in the bag for about a day before being added to phage buffer.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2016
Sequencing FacilityUniversity of Pittsburgh Genomics and Proteomics Core Laboratories
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1063
Genome length (bp)76261
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorthwestern College
Annotation StatusIn GenBank
Plaque NotesChelms produces small plaques. Some give a clear appearance and others give a haloed appearance.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Chelms_1

Chelms_2

Chelms_3

Chelms_4
(ssDNA binding protein)

Chelms_5

Chelms_6

Chelms_7

Chelms_8
(DNA primase)

Chelms_9

Chelms_10
(terminase large subunit)

Chelms_11
(portal protein)

Chelms_12
(hydrolase)

Chelms_13

Chelms_14
(capsid maturation protease)

Chelms_15
(scaffolding protein)

Chelms_16
(major capsid protein)

Chelms_17

Chelms_18

Chelms_19

Chelms_20

Chelms_21

Chelms_22

Chelms_23
(major tail protein)

Chelms_25
(tail assembly chaperone)

Chelms_26
(tail assembly chaperone)

Chelms_27
(tape measure protein)

Chelms_28
(minor tail protein)

Chelms_29
(minor tail protein)

Chelms_30
(minor tail protein)

Chelms_31

Chelms_32

Chelms_33

Chelms_34
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Chelms_35
(lysin A, protease domain)

Chelms_36

Chelms_37

Chelms_38

Chelms_39

Chelms_40
(exonuclease)

Chelms_41

Chelms_42

Chelms_43

Chelms_44

Chelms_45

Chelms_46
(DNA methylase)

Chelms_47

Chelms_48

Chelms_49

Chelms_50
(lysin B)

Chelms_51
(MazG-like nucleotide pyrophosphohydrolase)

Chelms_52
(RusA-like resolvase)

Chelms_53

Chelms_54
(DNA polymerase III sliding clamp)

Chelms_55

Chelms_56

Chelms_57
(helix-turn-helix DNA binding domain protein)

Chelms_58

Chelms_59
(DNA helicase)

Chelms_60

Chelms_61

Chelms_62

Chelms_63
(helix-turn-helix DNA binding domain protein)

Chelms_64

Chelms_65

Chelms_66

Chelms_67

Chelms_68

Chelms_69

Chelms_70

Chelms_71

Chelms_72

Chelms_73

Chelms_74

Chelms_75

Chelms_76

Chelms_77

Chelms_78

Chelms_79

Chelms_80

Chelms_81

Chelms_82

Chelms_83
(HNH endonuclease)

Chelms_84

Chelms_85

Chelms_86

Chelms_87

Chelms_88

Chelms_89
(CobT-like cobalamin biosynthesis protein)

Chelms_90
(AAA-ATPase)

Chelms_91

Chelms_92

Chelms_93

Chelms_94

Chelms_95

Chelms_96

Chelms_97

Chelms_98

Chelms_99

Chelms_100

Chelms_101

Chelms_102

Chelms_103

Chelms_104

Chelms_105

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801733
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3x10^10 pfu/mL
Date of SEA Lysate Titering Oct 26, 2016
Pitt Freezer Box# 42
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload