Detailed Information for Phage Chelms |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Cassie Helmer |
Year Found | 2016 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 40.4445 N, 79.9615 W Map |
Discovery Notes | The soil sample was collected from the grass area outside Braierl Recreation Center in the Peterson Event Center. The coordinates for where the soil was collected are 40.4445 N and 79.9615 W. The sample was taken from the edge of where the grass was growing next to a large block of stone. The soil was dry and had roots running through it. It was taken from the top layer of dirt. The soil was collected at 3;30 PM. The temperature was 75ºF and it was in the bag for about a day before being added to phage buffer. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 15, 2016 |
Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1063 |
Genome length (bp) | 76261 |
Character of genome ends | Direct Terminal Repeat |
Direct Terminal Repeat Length | 191 bp |
GC Content | 58.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CS |
Subcluster | CS2 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Northwestern College |
Annotation Status | In GenBank |
Plaque Notes | Chelms produces small plaques. Some give a clear appearance and others give a haloed appearance. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK801733 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
3x10^10 pfu/mL |
Date of SEA Lysate Titering |
Oct 26, 2016 |
Pitt Freezer Box# |
42 |
Pitt Freezer Box Grid# |
C5 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |