Gordonia phage Neoevie
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Detailed Information for Phage Neoevie
Discovery Information
Isolation HostGordonia terrae 3612
Found ByChelsea Smith
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4404 N, 79.959 W Map
Discovery NotesDiscovered from direct isolation techniques. Only one enriched isolation test was performed, but no plaques resulted from those methods.
Naming NotesThe meaning of "Neoevie" is "new life." Not only is the sequence of each phage finding new understanding of life for researchers, but I see this project as the beginning of a new phase of life for myself.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage266
Genome length (bp)73353
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length161 bp
GC Content59.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS3
Cluster Life CycleLytic
Lysogeny NotesThe phage appears to be a lytic phage.
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesPlaque sizes seems to vary from 1mm-3mm in diameter.
Has been Phamerated?Yes
Gene List

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Neoevie_1

Neoevie_2

Neoevie_3

Neoevie_4

Neoevie_5
(DNA primase)

Neoevie_6

Neoevie_7
(terminase)

Neoevie_8
(portal protein)

Neoevie_9

Neoevie_10

Neoevie_11
(capsid maturation protease)

Neoevie_12
(capsid decoration protein)

Neoevie_13
(major capsid protein)

Neoevie_14

Neoevie_15

Neoevie_16
(head-to-tail adaptor)

Neoevie_17

Neoevie_18

Neoevie_19

Neoevie_20
(major tail protein)

Neoevie_22
(tail assembly chaperone)

Neoevie_23
(tail assembly chaperone)

Neoevie_24
(tape measure protein)

Neoevie_25
(minor tail protein)

Neoevie_26
(minor tail protein)

Neoevie_27
(minor tail protein)

Neoevie_28

Neoevie_29

Neoevie_30

Neoevie_31

Neoevie_32

Neoevie_33

Neoevie_34

Neoevie_35
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Neoevie_36
(lysin A, protease M23 domain)

Neoevie_37

Neoevie_38

Neoevie_39

Neoevie_40

Neoevie_41
(Cas4 family exonuclease)

Neoevie_42

Neoevie_43

Neoevie_44

Neoevie_45

Neoevie_46
(DNA methylase)

Neoevie_47

Neoevie_48

Neoevie_49
(MazG-like nucleotide pyrophosphohydrolase)

Neoevie_50
(RusA-like resolvase)

Neoevie_51

Neoevie_52
(DNA polymerase III sliding clamp)

Neoevie_53

Neoevie_54
(DNA polymerase III subunit)

Neoevie_55
(helix-turn-helix DNA binding protein)

Neoevie_56

Neoevie_57
(DNA helicase)

Neoevie_58

Neoevie_59

Neoevie_60

Neoevie_61
(helix-turn-helix DNA binding protein)

Neoevie_62

Neoevie_63

Neoevie_64

Neoevie_65

Neoevie_66

Neoevie_67

Neoevie_68

Neoevie_69

Neoevie_70

Neoevie_71

Neoevie_72

Neoevie_73

Neoevie_74

Neoevie_75

Neoevie_76

Neoevie_77

Neoevie_78

Neoevie_79
(CobT-like cobalamin biosynthesis protein)

Neoevie_80
(CobT-like cobalamin biosynthesis protein)

Neoevie_81

Neoevie_82

Neoevie_83

Neoevie_84

Neoevie_85

Neoevie_86

Neoevie_87

Neoevie_88

Neoevie_89

Neoevie_90

Neoevie_91

Neoevie_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK450436
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.7*10^9pfu/mL
Date of SEA Lysate Titering Oct 26, 2016
Pitt Freezer Box# 42
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload