Gordonia phage Beaver
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Detailed Information for Phage Beaver
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByCarlee Hutchins, Griffin Hopkins, Tiffany Knowles
Year Found2018
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.1762 N, 93.0983 W Map
Discovery NotesDiscovered near local dam outpour on rocks near the water (5ft from water flow) on sunny/partly cloudy, 92*F day
Naming NotesWe chose the name Beaver because Beavers build dams and we found our phage near a dam.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 29, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage138
Genome length (bp)77394
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.9%
Sequencing NotesThis genome was sequenced as part of a deliberately mixed sample (with SheckWes), and determined to belong to phage Beaver based on restriction digest patterns.
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionOuachita Baptist University
Annotation StatusIn GenBank
Plaque Notes1mm diameter plaques, typically clean entire plates until the -3 dilution in serial dilutions
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Beaver_1

Beaver_2

Beaver_3

Beaver_4

Beaver_5

Beaver_6

Beaver_7

Beaver_8
(DNA primase)

Beaver_9

Beaver_10
(terminase)

Beaver_11
(portal protein)

Beaver_12
(minor tail protein)

Beaver_13

Beaver_14
(capsid maturation protease)

Beaver_15
(capsid decoration protein)

Beaver_16
(major capsid protein)

Beaver_17

Beaver_18

Beaver_19
(head-to-tail adaptor)

Beaver_20

Beaver_21

Beaver_22

Beaver_23
(major tail protein)

Beaver_25
(tail assembly chaperone)

Beaver_26
(tail assembly chaperone)

Beaver_27
(tape measure protein)

Beaver_28
(minor tail protein)

Beaver_29
(minor tail protein)

Beaver_30
(minor tail protein)

Beaver_31

Beaver_32

Beaver_33

Beaver_34

Beaver_35

Beaver_36

Beaver_37

Beaver_38
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Beaver_39
(lysin A, protease M23 domain)

Beaver_40

Beaver_41

Beaver_42

Beaver_43

Beaver_44
(Cas4 family exonuclease)

Beaver_45
(WhiB family transcription factor)

Beaver_46

Beaver_47

Beaver_48

Beaver_49

Beaver_50
(DNA methylase)

Beaver_51

Beaver_52

Beaver_53
(lysin B)

Beaver_54
(MazG-like nucleotide pyrophosphohydrolase)

Beaver_55

Beaver_56
(RusA-like resolvase)

Beaver_57

Beaver_58
(DNA polymerase III sliding clamp)

Beaver_59
(DNA polymerase III subunit)

Beaver_60
(helix-turn-helix DNA binding protein)

Beaver_61

Beaver_62
(DNA helicase)

Beaver_63

Beaver_64

Beaver_65

Beaver_66
(helix-turn-helix DNA binding protein)

Beaver_67

Beaver_68

Beaver_69

Beaver_70

Beaver_71

Beaver_72

Beaver_73

Beaver_74

Beaver_75

Beaver_76

Beaver_77

Beaver_78

Beaver_79

Beaver_80

Beaver_81

Beaver_82

Beaver_83

Beaver_84

Beaver_85

Beaver_86

Beaver_87

Beaver_88

Beaver_89

Beaver_90
(CobT-like cobalamin biosynthesis protein)

Beaver_91

Beaver_92
(AAA-ATPase)

Beaver_93

Beaver_94

Beaver_95

Beaver_96

Beaver_97

Beaver_98

Beaver_99

Beaver_100

Beaver_101

Beaver_102

Beaver_103

Beaver_104

Beaver_105

Beaver_106

Beaver_107

Beaver_108

Beaver_109

Beaver_110

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK967390
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.26x10^9 pfu/mL
Pitt Freezer Box# 78
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload