Gordonia phage Sam12
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Detailed Information for Phage Sam12
Discovery Information
Isolation HostGordonia terrae 3612
Former namesSH2022
Found BySamantha Haubner, Devesh Malik
Year Found2019
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.436389 N, 79.952778 W Map
Discovery NotesThe soil sample was collected on the side of Dawson Street in Pittsburgh PA around 8:30pm on 09/02. The soil was dark in color and damp. While it was cool weather conditions when the soil was collected, it had rained for a majority of the night before. There was a moderate amount of vegetation surrounding the soil. Soil was transported to a refrigerator within 5 minutes of collection and was stored there for 12 hours.
Naming Notesname and favorite number.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1644
Genome length (bp)75280
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Lysogeny NotesPhage titer determined to be 1.0x10^11 pfu/mL.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are small (diameter less than 2mm), circular, and clear in appearance.
Has been Phamerated?Yes
Gene List

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Sam12_1

Sam12_2

Sam12_3

Sam12_4
(dna binding protein)

Sam12_5

Sam12_6

Sam12_7

Sam12_8
(DNA primase)

Sam12_9
(terminase, small subunit)

Sam12_10
(terminase, large subunit)

Sam12_11
(portal protein)

Sam12_12
(hydrolase)

Sam12_13

Sam12_14
(capsid maturation protease)

Sam12_15
(scaffolding protein)

Sam12_16
(major capsid protein)

Sam12_17

Sam12_18

Sam12_19
(head-to-tail adaptor)

Sam12_20

Sam12_21

Sam12_22

Sam12_23
(major tail protein)

Sam12_25
(tail assembly chaperone)

Sam12_26
(tail assembly chaperone)

Sam12_27
(tape measure protein)

Sam12_28
(minor tail protein)

Sam12_29
(minor tail protein)

Sam12_30
(minor tail protein)

Sam12_31

Sam12_32

Sam12_33

Sam12_34
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Sam12_35
(lysin A, protease M23 domain)

Sam12_36

Sam12_37

Sam12_38

Sam12_39

Sam12_40
(Cas4 family exonuclease)

Sam12_41
(WhiB family transcription factor)

Sam12_42

Sam12_43

Sam12_44

Sam12_45

Sam12_46
(DNA methylase)

Sam12_47

Sam12_48

Sam12_49
(lysin B)

Sam12_50
(MazG-like nucleotide pyrophosphohydrolase)

Sam12_51

Sam12_52
(RusA-like resolvase (endonuclease))

Sam12_53
(nucleoside deoxyribosyltransferase)

Sam12_54
(DNA polymerase III sliding clamp (Beta))

Sam12_55
(DnaQ-like (DNA polymerase III subunit))

Sam12_56
(helix-turn-helix DNA binding domain)

Sam12_57

Sam12_58
(DNA helicase)

Sam12_59

Sam12_60

Sam12_61

Sam12_62
(helix-turn-helix DNA binding domain)

Sam12_63

Sam12_64

Sam12_65

Sam12_66

Sam12_67

Sam12_68

Sam12_69

Sam12_70

Sam12_71

Sam12_72

Sam12_73

Sam12_74

Sam12_75

Sam12_76

Sam12_77

Sam12_78

Sam12_79

Sam12_80

Sam12_81
(HNH endonuclease)

Sam12_82

Sam12_83

Sam12_84

Sam12_85

Sam12_86

Sam12_87
(CobT-like cobalamin biosynthesis protein)

Sam12_88
(AAA-ATPase)

Sam12_89

Sam12_90

Sam12_91

Sam12_92

Sam12_93

Sam12_94

Sam12_95

Sam12_96

Sam12_97

Sam12_98

Sam12_99

Sam12_100

Sam12_101

Sam12_102

Sam12_103

Sam12_104

Sam12_105

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT889368
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 115
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload