Gordonia phage John316
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Detailed Information for Phage John316
Discovery Information
Isolation HostGordonia terrae 3612
Found BySamantha Blum
Year Found2019
Location FoundRock Valley, IA United States
Finding InstitutionNorthwestern College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates43.207134 N, 96.293091 W Map
Discovery NotesThe soil was taken from a flower garden right before frost.
Naming Notes"For God so loved the world that he gave his one and only Son, that whoever believes in him shall not perish but have eternal life."- John 3:16

It is a cliche Bible verse, but most Christians find this verse to be at the root of their faith.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 21, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage473
Genome length (bp)77221
Character of genome endsDirect Terminal Repeat
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorthwestern College
Annotation StatusIn GenBank
Plaque NotesThe plaques are small and have rough edges. They are not turbid. The plaques do not increase in size after a longer incubation period.
MorphotypeSiphoviridae
Number of Genes110
Number of tRNAs1
Has been Phamerated?Yes
Gene List

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John316_1

John316_2

John316_3

John316_4

John316_5

John316_6

John316_7

John316_8
(DNA primase/polymerase)

John316_9
(terminase, small subunit)

John316_10
(terminase, large subunit)

John316_11
(portal protein)

John316_12
(hydrolase)

John316_13

John316_14
(capsid maturation protease)

John316_15
(scaffolding protein)

John316_16
(major capsid protein)

John316_17

John316_18

John316_19
(head-to-tail adaptor)

John316_20

John316_21

John316_22

John316_23
(major tail protein)

John316_25
(tail assembly chaperone)

John316_26
(tail assembly chaperone)

John316_27
(tape measure protein)

John316_28
(minor tail protein)

John316_29
(minor tail protein)

John316_30
(minor tail protein)

John316_31

John316_32

John316_33

John316_34

John316_35

John316_36
(holin)

John316_37

John316_38
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

John316_39
(lysin A, protease M23 domain)

John316_40
(membrane protein)

John316_41
(membrane protein)

John316_42

John316_43
(membrane protein)

John316_44
(RecB-like Exonuclease/helicase)

John316_45
(WhiB family transcription factor)

John316_46

John316_47

John316_48
(membrane protein)

John316_49

John316_50
(DNA Methylase)

John316_51

John316_52

John316_53
(lysin B)

John316_54
(MazG-like nucleotide pyrophosphohydrolase)

John316_55

John316_56
(RusA-like resolvase (endonuclease))

John316_57

John316_58
(DNA polymerase III sliding clamp (Beta))

John316_59
(DnaQ-like (DNA polymerase III subunit))

John316_60
(helix-turn-helix DNA binding domain)

John316_61

John316_62
(DNA helicase)

John316_63

John316_64

John316_65

John316_66
(helix-turn-helix DNA binding domain)

John316_67

John316_68

John316_69

John316_70

John316_71

John316_72

John316_73

John316_74

John316_75

John316_76

John316_77

John316_78

John316_79

John316_80

John316_81

John316_82

John316_83

John316_84

John316_85

John316_86

John316_87

John316_88

John316_89

John316_90

John316_91
(CobT-like cobalamin biosynthesis protein)

John316_92
(AAA-ATPase)

John316_93

John316_94

John316_95

John316_96

John316_97

John316_98

John316_99

John316_100

John316_101

John316_102

John316_103

John316_104

John316_105

John316_106

John316_107

John316_108

John316_109

John316_110

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN945905
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload