Gordonia phage Msay19
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Detailed Information for Phage Msay19
Discovery Information
Isolation HostGordonia terrae 3612
Found ByRobert Sayce
Year Found2017
Location FoundPITTSBURGH, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature-11°C
GPS Coordinates40.444444 N, 79.954722 W Map
Discovery NotesThis phage was discovered in front of the cathedral of learning on 01/08/2017, the temperature at time of collection was 12 degrees Fahrenheit. This phage came from a sample collected by Kristin Link.
Naming NotesThis phage was named using the first letter of someone I miss dearly and the first letters of my last name, and 19 being my favorite number.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 22, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1420
Genome length (bp)77513
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesThis forms a small relatively clear plaque with cloudy plaques found on alternate hosts
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Msay19_1

Msay19_2

Msay19_3

Msay19_4

Msay19_5

Msay19_6

Msay19_7

Msay19_8

Msay19_9
(DNA primase)

Msay19_10

Msay19_11
(terminase)

Msay19_12
(portal protein)

Msay19_13
(minor tail protein)

Msay19_14

Msay19_15
(capsid maturation protease)

Msay19_16
(capsid decoration protein)

Msay19_17
(major capsid protein)

Msay19_18

Msay19_19

Msay19_20
(head-to-tail adaptor)

Msay19_21

Msay19_22

Msay19_23

Msay19_24
(major tail protein)

Msay19_26
(tail assembly chaperone)

Msay19_27
(tail assembly chaperone)

Msay19_28
(tape measure protein)

Msay19_29
(minor tail protein)

Msay19_30
(minor tail protein)

Msay19_31
(minor tail protein)

Msay19_32

Msay19_33

Msay19_34

Msay19_35

Msay19_36

Msay19_37

Msay19_38

Msay19_39
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Msay19_40
(lysin A, protease M23 domain)

Msay19_41

Msay19_42

Msay19_43

Msay19_44

Msay19_45
(Cas4 family exonuclease)

Msay19_46

Msay19_47

Msay19_48

Msay19_49

Msay19_50

Msay19_51
(DNA methylase)

Msay19_52

Msay19_53

Msay19_54
(lysin B)

Msay19_55
(MazG-like nucleotide pyrophosphohydrolase)

Msay19_56

Msay19_57
(RusA-like resolvase)

Msay19_58

Msay19_59
(DNA polymerase III sliding clamp)

Msay19_60

Msay19_61
(DNA polymerase III subunit)

Msay19_62
(helix-turn-helix DNA binding protein)

Msay19_63

Msay19_64
(DNA helicase)

Msay19_65

Msay19_66

Msay19_67

Msay19_68
(helix-turn-helix DNA binding protein)

Msay19_69

Msay19_70

Msay19_71

Msay19_72

Msay19_73

Msay19_74

Msay19_75

Msay19_76

Msay19_77

Msay19_78

Msay19_79

Msay19_80

Msay19_81

Msay19_82

Msay19_83

Msay19_84

Msay19_85

Msay19_86
(HNH endonuclease)

Msay19_87

Msay19_88

Msay19_89

Msay19_90

Msay19_91

Msay19_92
(CobT-like cobalamin biosynthesis protein)

Msay19_93
(AAA-ATPase)

Msay19_94

Msay19_95

Msay19_96

Msay19_97

Msay19_98

Msay19_99

Msay19_100

Msay19_101

Msay19_102

Msay19_103

Msay19_104

Msay19_105

Msay19_106

Msay19_107

Msay19_108

Msay19_109

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK433261
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^10pfu/mL
Date of SEA Lysate Titering Mar 1, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# A9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload