| Detailed Information for Phage BigChungus |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Sean Glasshoff and Austin Herold |
| Year Found | 2019 |
| Location Found | Omaha, NE United States |
| Finding Institution | University of Nebraska-Lincoln |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 41.2097 N, 96.2161 W Map |
| Discovery Notes | BigChungus was discovered in the soil of Sean's front yard garden bed at his house in Omaha, NE. It was obtained through direct isolation. |
| Naming Notes | The name comes from the main character of a popular Sony PlayStation video game. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1724 |
| Genome length (bp) | 47166 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CGGCGGTAGGCTT |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Nebraska-Lincoln |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques of BigChungus are completely clear with uniform edges. They measure approximately 2 mm in diameter. |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT776810 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
103 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
