Streptomyces phage Diane
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Detailed Information for Phage Diane
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByOlivia Jones
Year Found2016
Location FoundFrisco, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.122546 N, 96.789546 W Map
Discovery NotesI collected the soil on August 27th, 2016 at 3:32pm. The temperature was 86 degrees Fahrenheit. The soil was moist, dark, and was under a small tree.
Naming NotesDiane is my middle name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 30, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage200
Genome length (bp)50483
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Other Cluster Members

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Aaronocolus (BD1)

Asten (BD1)

BarryBee (BD1)

BeardedLady (BD1)

Bovely (BD1)

Brataylor (BD1)

BryanRecycles (BD1)

Caliburn (BD1)

Celeste (BD1)

Chucky (BD1)

Danzina (BD1)

Dattran (BD1)

Dwayne (BD1)

Eddasa (BD1)

Ejemplo (BD1)

Emaanora (BD1)

EnochSoames (BD1)

Esperer (BD1)

Goby (BD1)

Godpower (BD1)

Hippo (BD1)

Hydra (BD1)

Indigo (BD1)

Izzy (BD1)

Jash (BD1)

Katalie (BD1)

Lannister (BD1)

Legacy (BD1)

Leviticus (BD1)

Lika (BD1)

Lorelei (BD1)

Maneekul (BD1)

Nabi (BD1)

Nanodon (BD1)

Nerdos (BD1)

Oliynyk (BD1)

Ozzie (BD1)

OzzyJ (BD1)

Phettuccine (BD1)

Rana (BD1)

RedBear (BD1)

Rusticus (BD1)

SarahRose (BD1)

Snorlax (BD1)

South40 (BD1)

Sujidade (BD1)

SunsetPointe (BD1)

TagePhighter (BD1)

Toma (BD1)

Triste (BD1)

TuanPN (BD1)

Unstoppable (BD1)

Werner (BD1)

Whatever (BD1)

Yasdnil (BD1)

Zemlya (BD1)

Alvy (BD2)

Amethyst (BD2)

Andris (BD2)

Animus (BD2)

BartholomewSD (BD2)

Bowden (BD2)

Caelum (BD2)

Daudau (BD2)

Diane (BD2)

ELB20 (BD2)

GirlDinner (BD2)

Haizum (BD2)

Hank144 (BD2)

Issmi (BD2)

Janus (BD2)

Jevington (BD2)

Loofah (BD2)

Marav (BD2)

Nishikigoi (BD2)

Omar (BD2)

Pablito (BD2)

Paedore (BD2)

Paolo (BD2)

Puginator (BD2)

R4 (BD2)

SqueakyClean (BD2)

Superstar (BD2)

Tefunt (BD2)

Thestral (BD2)

TinaBelcher (BD2)

Triumph (BD2)

TrvxScott (BD2)

Zainub (BD2)

Alsaber (BD3)

Amela (BD3)

Celery (BD3)

Conan (BD3)

Dexers (BD3)

ElGato (BD3)

Kaine (BD3)

Pavo (BD3)

phiCAM (BD3)

Provolone (BD3)

Saftant (BD3)

Speedwell (BD3)

Vanseggelen (BD3)

Verabelle (BD3)

Verse (BD3)

Yosif (BD3)

BroPlease (BD4)

GreenWeasel (BD4)

phiHau3 (BD4)

StrepC (BD5)

Celia (BD6)

Itza (BD6)

Urza (BD6)

VieEnRose (BD6)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques are on average 0.1cm in diameter, are clear, and circular.
MorphotypeSiphoviridae
Number of Genes79
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Diane_1

Diane_2
(HNH endonuclease)

Diane_3

Diane_4
(thioredoxin)

Diane_5

Diane_6
(terminase large subunit)

Diane_7
(portal protein)

Diane_8

Diane_9
(scaffolding protein)

Diane_10
(major capsid protein)

Diane_11
(head-to-tail adaptor)

Diane_12
(head-to-tail stopper)

Diane_13

Diane_14
(tail terminator)

Diane_15
(major tail protein)

Diane_16
(tail assembly chaperone)

Diane_17
(tail assembly chaperone)

Diane_18
(tape measure protein)

Diane_19
(minor tail protein)

Diane_20
(minor tail protein)

Diane_21

Diane_22
(minor tail protein)

Diane_23

Diane_24
(minor tail protein)

Diane_25

Diane_26
(endolysin)

Diane_27

Diane_28
(DNA binding protein)

Diane_29
(immunity repressor)

Diane_30

Diane_31
(exonuclease)

Diane_32

Diane_33

Diane_34
(deoxycytidylate deaminase)

Diane_35
(DnaB-like dsDNA helicase)

Diane_36
(holliday junction resolvase)

Diane_37

Diane_38
(DNA primase)

Diane_39
(DNA primase)

Diane_40

Diane_41

Diane_42

Diane_43
(MRE11 double-strand break endo/exonuclease)

Diane_44

Diane_45
(DNA binding protein)

Diane_46

Diane_47
(ribonucleotide reductase)

Diane_48

Diane_49

Diane_50
(DNA binding protein)

Diane_51
(serine integrase)

Diane_52

Diane_53

Diane_54
(ThyX-like thymidylate synthase)

Diane_55

Diane_56

Diane_57

Diane_58

Diane_59

Diane_60

Diane_61

Diane_62

Diane_63
(deoxynucleoside monophosphate kinase)

Diane_64

Diane_65

Diane_66

Diane_67

Diane_68

Diane_69
(OCR-like antirestriction protein)

Diane_70

Diane_71

Diane_72

Diane_73

Diane_74

Diane_75

Diane_76
(acetyltransferase)

Diane_77

Diane_78

Diane_79

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF766046
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.0X10^8
Date of SEA Lysate Titering Oct 19, 2016
Pitt Freezer Box# 36
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload