Gordonia phage Ekhein
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Detailed Information for Phage Ekhein
Discovery Information
Isolation HostGordonia terrae 3612
Found ByTrezalka Budinsky
Year Found2019
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.440378 N, 79.955711 W Map
Discovery NotesThe soil sample was taken from the Oakland Avenue Farm, which is located on Oakland Avenue across from Bouquet Gardens. The soil was partly dry, due to the fact that we have not had rain in a while. The soil was not compact, therefore, very loose and easy to collect. The weather at the time of collection was extremely hot, dry, and sunny. The area of collection was located specifically in a plot of garden soil, which was a wide, open space.
Naming NotesThe ancient Greek word for "restrain" - our phage's plaques are restrained to a tiny area. It is also the origin of the name Hektor - a famous Trojan hero from the Iliad.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage675
Genome length (bp)76612
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversidad Autónoma de Nuevo León
Annotation StatusIn GenBank
Plaque NotesSmall clear plaques - ranging from 0.5 to 2.0mm in diameter.
MorphotypeSiphoviridae
Number of Genes108
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Ekhein_1

Ekhein_2

Ekhein_3

Ekhein_4

Ekhein_5

Ekhein_6

Ekhein_7

Ekhein_8
(DNA primase)

Ekhein_9

Ekhein_10
(terminase)

Ekhein_11
(portal protein)

Ekhein_12
(hydrolase)

Ekhein_13

Ekhein_14
(capsid maturation protease)

Ekhein_15
(capsid decoration protein)

Ekhein_16
(major capsid protein)

Ekhein_17

Ekhein_18

Ekhein_19
(head-to-tail adaptor)

Ekhein_20

Ekhein_21

Ekhein_22

Ekhein_23
(major tail protein)

Ekhein_24 — tRNA
(tRNA-Asn(gtt))

Ekhein_25
(tail assembly chaperone)

Ekhein_26
(tail assembly chaperone)

Ekhein_27
(tape measure protein)

Ekhein_28
(minor tail protein)

Ekhein_29
(minor tail protein)

Ekhein_30
(minor tail protein)

Ekhein_31

Ekhein_32
(holin)

Ekhein_33

Ekhein_34
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Ekhein_35
(lysin A, protease M23 domain)

Ekhein_37

Ekhein_38

Ekhein_39

Ekhein_40

Ekhein_41
(Cas4 family exonuclease)

Ekhein_42
(WhiB family transcription factor)

Ekhein_43

Ekhein_44

Ekhein_45

Ekhein_46

Ekhein_47
(DNA methylase)

Ekhein_48

Ekhein_49

Ekhein_50
(lysin B)

Ekhein_51
(MazG-like nucleotide pyrophosphohydrolase)

Ekhein_52

Ekhein_53
(RusA-like resolvase)

Ekhein_54

Ekhein_55
(DNA polymerase III sliding clamp)

Ekhein_56
(DnaQ-like DNA polymerase III subunit)

Ekhein_57
(helix-turn-helix DNA binding protein)

Ekhein_58

Ekhein_59
(DNA helicase)

Ekhein_60

Ekhein_61

Ekhein_62

Ekhein_63
(helix-turn-helix DNA binding protein)

Ekhein_64

Ekhein_65

Ekhein_66

Ekhein_67

Ekhein_68

Ekhein_69

Ekhein_70

Ekhein_71

Ekhein_72

Ekhein_73

Ekhein_74

Ekhein_75

Ekhein_76

Ekhein_77

Ekhein_78

Ekhein_79

Ekhein_80

Ekhein_81

Ekhein_82

Ekhein_83

Ekhein_84
(HNH endonuclease)

Ekhein_85

Ekhein_86

Ekhein_87

Ekhein_88

Ekhein_89

Ekhein_90
(CobT-like cobalamin biosynthesis protein)

Ekhein_91
(AAA-ATPase)

Ekhein_92

Ekhein_93

Ekhein_94

Ekhein_95

Ekhein_96

Ekhein_97

Ekhein_98

Ekhein_99

Ekhein_100

Ekhein_102

Ekhein_103

Ekhein_104

Ekhein_105

Ekhein_106

Ekhein_107

Ekhein_108

Ekhein_109

Ekhein_110

Ekhein_111

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW507132
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 114
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload