| Detailed Information for Phage Elezi |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Emanuela Elezi |
| Year Found | 2019 |
| Location Found | Waterbury, CT United States |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.554167 N, 72.959722 W Map |
| Discovery Notes | The Phage was collected near my house. The soil collected was located in between two trees and the grass was growing around it. There was not much sun exposure. The depth of the sample collection was 1-3cm deep. The soil sample was dark in color and moist. It rained a couple of days before the sample collection, 09/04/19. The air humidity was around 80%. (650ft of elevation) |
| Naming Notes | I named the phage after my last name |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1377 |
| Genome length (bp) | 43471 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Southern Connecticut State University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques from the phage are relatively big, their diameter is around 5mm. The plaques are temperate, there is a clearing in the center and as it reaches the outer side a cloudy ring is formed.
The average width of the capsid is 61.689nm and its average length is 61.394, the average length of the tail is 122.192nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT639653 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8x10^10 pfu/ml |
| Pitt Freezer Box# |
102 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
