| Detailed Information for Phage Exile | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus NRRL B-14730 |
| Former names | Twizzle |
| Found By | Soniya Agarwal |
| Year Found | 2023 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 13°C |
| GPS Coordinates | 45.270408 N, 75.898157 W Map |
| Discovery Notes | Soil was collected from under a young-tree on the evening of September 20th, 2023 in Stittsville, Ottawa. The soil was damp, dark and was collected about eight centimetres from the surface. |
| Naming Notes | Phage was named after one of my favourite songs from the Folklore album by Taylor Swift. Phage also caused lots of trouble during purification and amplification, and thus, deserved to be "exiled". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1095 |
| Genome length (bp) | 44132 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 65.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage is lysogenic, forming plaques with cloudy centres. |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) Sooty (AZ5) SpecialK (AZ5) |
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | Plaque are relatively small in size, and are turbid in the centre and then sharply turn clear at the periphery. Plaques resemble a "bullseye" configuration. |
| Morphotype | Siphoviridae |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewExile_1 Exile_2 Exile_3 Exile_4 Exile_5 Exile_6 Exile_7 Exile_8 Exile_9 Exile_10 Exile_11 Exile_12 Exile_13 Exile_14 Exile_15 Exile_16 Exile_17 Exile_18 Exile_19 Exile_20 Exile_21 Exile_22 Exile_23 Exile_24 Exile_25 Exile_26 Exile_27 Exile_28 Exile_29 Exile_30 Exile_31 Exile_32 Exile_33 Exile_34 Exile_35 Exile_36 Exile_37 Exile_38 Exile_39 Exile_40 Exile_41 Exile_42 Exile_43 Exile_44 Exile_45 Exile_46 Exile_47 Exile_48 Exile_49 Exile_50 Exile_51 Exile_52 Exile_53 Exile_54 Exile_55 Exile_56 Exile_57 Exile_58 Exile_59 Exile_60 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 176 |
| Pitt Freezer Box Grid# | C4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
