| Detailed Information for Phage Gambol |
| Discovery Information |
| Isolation Host | Curtobacterium flaccumfaciens NRRL B-729 SEA |
| Found By | Theresa Chiu |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.441836 N, 80.008586 W Map |
| Discovery Notes | Under a tree by a sign that says I-279 at Point Park. |
| Naming Notes | Named after Blake Belladonna's (RWBY) weapon.
Annotated by T. Chiu. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 1, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 10950 |
| Genome length (bp) | 40063 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAACGGCAT |
| GC Content | 70.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Large, circular plaques |
| Number of Genes | 62 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ868992 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.2*10^10 |
| Pitt Freezer Box# |
219 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| GenBank File for Phamerator | Download |
