| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Manishika Suresh |
| Year Found | 2021 |
| Location Found | Denton, TX United States of America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.212239 N, 97.146985 W Map |
| Discovery Notes | I collected soil from (33.212239°N, 97.146985°W) in southeastern Denton, Texas at 7:40 pm. The temperature was about 91°F and was extremely dry due to a few days of only clear, sunny skies. The location is around a tree near a sidewalk in front of Sage Hall. I dug about the top of 1 inch of soil between the roots of the tree and placed it in a plastic Ziploc bag along with the spoon I used to dig the soil. |
| Naming Notes | I am a student at the University of North Texas, so the "Green" in the name represents that. Since this is my freshman year, I got to move in early to experience First Flight Week, and through that, I met my friend group, and we lovingly refer to ourselves as the "Weasels", giving me the 2nd half of the phage name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 6, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 206 |
| Genome length (bp) | 50778 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAGCCGTGAT |
| GC Content | 67.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | the plaques are all 1mm in diameter, they all have a clear center and have a pretty clear, but cloudier than the center ring which creates the diameter |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON526974 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2*10^8 pfu/mL |
| Pitt Freezer Box# |
143 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
