The Actinobacteriophage Database

Gordonia phage Hexbug

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Detailed Information for Phage Hexbug
Discovery Information
Isolation Host	Gordonia rubripertincta NRRL B-16540
Found By	Daniel Barszczak
Year Found	2021
Location Found	MA United States
Finding Institution	Tufts University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	Not entered
GPS Coordinates	42.412895 N, 71.13305 W Map
Discovery Notes	The location information was scrambled between two samples but will be clarified soon after redoing part of the procedure.
Naming Notes	They have hexagon heads and are bugs (viruses). They also look like Hexbug toy tracks.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 18, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1038
Genome length (bp)	47190
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGTAGGCAT
GC Content	63.3%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	CT
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Agatha AikoCarson Amok AndPeggy Axym Azira Balloona Bavilard BigChungus BillDoor Biskit Blondies Bubble Burnsey Button Buttrmlkdreams CanesSauce CaramelLatte Carsonalex CherryonLim ChickenTender ChocoMunchkin Cleo CocoaPuff Cozz Dre3 Elinal Eliott ElJefes Emalyn Feastonyeet Fribs8 Gibbous GiKK GoldHunter GTE2 Hexbug HippoPololi Horseradish Jamzy KayGee Lauer MAnor Margaret MaVan Mayweather MScarn MunkgeeRoachy Nibbles Nina Nodigi Orla Pons PotPie PsychoKiller Quasar RanchParmCat RedBaron SheckWes SketchMex Socotra Sopespian Starburst SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Typhonomachy Vine Wolfwood Yakult Yarn Yucky Yummy Zareef
Annotating Institution	Tufts University
Annotation Status	In GenBank
Number of Genes	73
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Hexbug_1 (terminase small subunit) Hexbug_2 Hexbug_3 Hexbug_4 Hexbug_5 (terminase large subunit) Hexbug_6 (portal protein) Hexbug_7 (capsid maturation protease) Hexbug_8 Hexbug_9 (scaffolding protein) Hexbug_10 (major capsid protein) Hexbug_11 (head-to-tail adaptor) Hexbug_12 (head-to-tail stopper) Hexbug_13 Hexbug_14 (tail terminator) Hexbug_15 (major tail protein) Hexbug_16 (tail assembly chaperone) Hexbug_17 (tail assembly chaperone) Hexbug_18 (tape measure protein) Hexbug_19 (minor tail protein) Hexbug_20 (minor tail protein) Hexbug_21 (minor tail protein) Hexbug_22 (lysin A, L-Ala-D-Glu peptidase domain) Hexbug_23 (lysin A, glycosyl hydrolase domain) Hexbug_24 (holin) Hexbug_25 Hexbug_26 Hexbug_27 Hexbug_28 Hexbug_29 Hexbug_30 Hexbug_31 (hydrolase) Hexbug_32 Hexbug_33 Hexbug_34 (hydrolase) Hexbug_35 Hexbug_36 Hexbug_37 Hexbug_38 Hexbug_39 Hexbug_40 (SprT-like protease) Hexbug_41 Hexbug_42 (helix-turn-helix DNA binding domain protein) Hexbug_43 (MerR-like helix-turn-helix DNA binding domain protein) Hexbug_44 Hexbug_45 (nuclease) Hexbug_46 Hexbug_47 (AAA-ATPase) Hexbug_48 Hexbug_49 (RecB-like exonuclease/helicase) Hexbug_50 Hexbug_51 (DNA polymerase I) Hexbug_52 (deoxycytidylate deaminase) Hexbug_53 (lysin B) Hexbug_54 (thymidylate synthase) Hexbug_55 Hexbug_56 Hexbug_57 Hexbug_58 (dUTPase) Hexbug_59 (thymidylate kinase) Hexbug_60 (helicase) Hexbug_61 Hexbug_62 Hexbug_63 (DNA primase/helicase) Hexbug_64 Hexbug_65 Hexbug_66 Hexbug_67 Hexbug_68 Hexbug_69 Hexbug_70 Hexbug_71 Hexbug_72 Hexbug_73
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	ON970609
Refseq Number	None yet
Published in Paper	Barszczak et al 2022, Microbiology Resource Announcements: Complete Genome Sequence of Gordonia rubripertincta Bacteriophage Hexbug Suggests Potential for a New CT Subcluster
Archiving Info
Archiving status	Archived
SEA Lysate Titer	1.18 * 10^9 pfu/ml
Pitt Freezer Box#	136
Pitt Freezer Box Grid#	A4
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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