Gordonia phage Horseradish
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Detailed Information for Phage Horseradish
Discovery Information
Isolation HostGordonia terrae 3612
Found ByGrace Hodgkin and Whitney St. Pierre
Year Found2022
Location FoundOld Town, ME United States of America
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates44.928847 N, 68.67177 W Map
Discovery NotesFrom composted soil from Roger's Farm in Orono, Maine; collected by Tom Molloy.
Naming NotesWe wanted to name it after a vegetable since it came from a compost sample from a farm.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage201
Genome length (bp)45764
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCTT
GC Content60.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCT
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesThis phage produces no lysogens.
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque Notes3.5 mm diameter, clear plaques- consistent with lytic phages
MorphotypeSiphoviridae
Number of Genes71
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Horseradish_1
(terminase small subunit)

Horseradish_2

Horseradish_3

Horseradish_4

Horseradish_5
(terminase large subunit)

Horseradish_6
(portal protein)

Horseradish_7

Horseradish_8
(scaffolding protein)

Horseradish_9
(major capsid protein)

Horseradish_10
(head-to-tail adaptor)

Horseradish_11
(head-to-tail stopper)

Horseradish_12

Horseradish_13
(tail terminator)

Horseradish_14
(major tail protein)

Horseradish_15
(tail assembly chaperone)

Horseradish_16
(tail assembly chaperone)

Horseradish_17
(tape measure protein)

Horseradish_18
(minor tail protein)

Horseradish_19
(minor tail protein)

Horseradish_20

Horseradish_21
(lysin A, L-Ala-D-Glu peptidase domain)

Horseradish_22
(lysin A, glycosyl hydrolase domain)

Horseradish_23
(holin)

Horseradish_24

Horseradish_25

Horseradish_26
(minor tail protein)

Horseradish_27

Horseradish_28
(minor tail protein)

Horseradish_29

Horseradish_30

Horseradish_31

Horseradish_32

Horseradish_33
(helix-turn-helix DNA binding domain protein)

Horseradish_34
(MerR-like helix-turn-helix DNA binding domain protein)

Horseradish_35

Horseradish_36

Horseradish_37
(holliday junction revolvase)

Horseradish_38
(peptidase)

Horseradish_39

Horseradish_40

Horseradish_41

Horseradish_42
(RecA superfamily ATPase)

Horseradish_43
(Cas4 family exonuclease)

Horseradish_44

Horseradish_45
(DNA polymerase I)

Horseradish_46
(deoxycytidylate deaminase)

Horseradish_47

Horseradish_48
(lysin B)

Horseradish_49

Horseradish_50
(thymidylate synthase)

Horseradish_51

Horseradish_52

Horseradish_53
(dUTPase)

Horseradish_54
(thymidylate kinase)

Horseradish_55
(DNA helicase)

Horseradish_56

Horseradish_57

Horseradish_58

Horseradish_59

Horseradish_60
(DNA primase/helicase)

Horseradish_61

Horseradish_62

Horseradish_63

Horseradish_64

Horseradish_65

Horseradish_66

Horseradish_67

Horseradish_68

Horseradish_69

Horseradish_70

Horseradish_71
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR159672
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 163
Pitt Freezer Box Grid# D9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload