Detailed Information for Phage Izzy |
Discovery Information |
Isolation Host | Streptomyces griseus ATCC 10137 |
Found By | Jessi Narvaez and Orooj Syed |
Year Found | 2014 |
Location Found | San Antonio, TX USA |
Finding Institution | University of North Texas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 29.590722 N, 98.993167 W Map |
Discovery Notes | Soil sample was taken from a backyard in San Antonio, TX. The air temperature was approximately 80 degrees F. It was collected on August 31st, 2014. This sample was adopted from Dhanya Prasad. |
Naming Notes | We named the phage Izzy because it is Jessi's cat's name and Orooj's favorite character on the TV show Grey's Anatomy. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 9, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 1360 |
Genome length (bp) | 50113 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGGGCAGTGAT |
GC Content | 65.9% |
Sequencing Notes | This DNA sample had a 47 bp deletion that was present in about 60% of the reads. The bases from 560 to 606 (inclusive) are the ones deleted. |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BD |
Subcluster | BD1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaques were approximately 3 mm in diameter. They were irregular circles with halos and were clear. |
Morphotype | Siphoviridae |
Number of Genes | 75 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KT184390 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
36 |
Pitt Freezer Box Grid# |
B5 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |