| Detailed Information for Phage Izzy |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Jessi Narvaez and Orooj Syed |
| Year Found | 2014 |
| Location Found | San Antonio, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.590722 N, 98.993167 W Map |
| Discovery Notes | Soil sample was taken from a backyard in San Antonio, TX. The air temperature was approximately 80 degrees F. It was collected on August 31st, 2014. This sample was adopted from Dhanya Prasad. |
| Naming Notes | We named the phage Izzy because it is Jessi's cat's name and Orooj's favorite character on the TV show Grey's Anatomy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1360 |
| Genome length (bp) | 50113 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| GC Content | 65.9% |
| Sequencing Notes | This DNA sample had a 47 bp deletion that was present in about 60% of the reads. The bases from 560 to 606 (inclusive) are the ones deleted. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were approximately 3 mm in diameter. They were irregular circles with halos and were clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT184390 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
B5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
