| Detailed Information for Phage Janus |
| Discovery Information |
| Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
| Found By | Jose Molina |
| Year Found | 2016 |
| Location Found | Dallas, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.730278 N, 96.888611 W Map |
| Discovery Notes | I collected the soil on August 27, 2016 at 7:25 p.m. The temperature at the time was 91 degrees Fahrenheit. I dug about 2 to 3 inches into the ground and collected it; the soil was quite moist and damp. |
| Naming Notes | I decided to name this phage Janus because Janus is the Greek god of beginnings, and I liked it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 7, 2017 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 77 |
| Genome length (bp) | 50632 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCGTGTCTT |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The average diameter for my phage was about 1mm. It was circular and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK392366 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.2x10^8 pfu/ml |
| Date of SEA Lysate Titering |
Nov 10, 2016 |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
