| Detailed Information for Phage LadyAstra | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Brooklyn Burgess |
| Year Found | 2024 |
| Location Found | Northridge, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.22487 N, 118.55811 W Map |
| Discovery Notes | Sample description: backyard of house, between flowers and tree Depth from which sample was taken: 5cm Air temperature if sample collected outdoors (in degrees Celsius): 36.67 Direct Plating or Enrichment: Enriched Enrichment protocol used (if any): Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: F24-1A Instructor: Dr. Daniel Teaching Assistant: Neel B. Undergraduate Assistant(s): Andy K. and Cindy V. Team #1 Name: Vicious Viruses Team #1 Members: Usha, Pranava, Alondra and David Team #2 Name: Phage Phreaks Team #2 Members: Brooklyn Burgess, Diego Sahagun, Kevin Zhang, Sara Piao Team #3 Name: Biohazard Baddies Team #3 Members: Noah Graham, Hannah Kang, Sophie Nuckowski, Sarah Bonver |
| Naming Notes | VVA chose Astra, and PP added the prefix of Lady. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 6948 |
| Genome length (bp) | 43408 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) Sooty (AZ5) SpecialK (AZ5) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: halo plaques Average size (and size range) diameter in mm: 3 mm, but ranges 1mm-3mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
