| Detailed Information for Phage LadyAstra | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Brooklyn Burgess |
| Year Found | 2024 |
| Location Found | Northridge, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.22487 N, 118.55811 W Map |
| Discovery Notes | Sample description: backyard of house, between flowers and tree Depth from which sample was taken: 5cm Air temperature if sample collected outdoors (in degrees Celsius): 36.67 Direct Plating or Enrichment: Enriched Enrichment protocol used (if any): Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: F24-1A Instructor: Dr. Daniel Teaching Assistant: Neel B. Undergraduate Assistant(s): Andy K. and Cindy V. Team #1 Name: Vicious Viruses Team #1 Members: Usha, Pranava, Alondra and David Team #2 Name: Phage Phreaks Team #2 Members: Brooklyn Burgess, Diego Sahagun, Kevin Zhang, Sara Piao Team #3 Name: Biohazard Baddies Team #3 Members: Noah Graham, Hannah Kang, Sophie Nuckowski, Sarah Bonver |
| Naming Notes | VVA chose Astra, and PP added the prefix of Lady. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 6948 |
| Genome length (bp) | 43408 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Shaffner (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) Sooty (AZ5) SpecialK (AZ5) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: halo plaques Average size (and size range) diameter in mm: 3 mm, but ranges 1mm-3mm |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLadyAstra_1 LadyAstra_2 LadyAstra_3 LadyAstra_4 LadyAstra_5 LadyAstra_6 LadyAstra_7 LadyAstra_8 LadyAstra_9 LadyAstra_10 LadyAstra_11 LadyAstra_12 LadyAstra_13 LadyAstra_14 LadyAstra_15 LadyAstra_16 LadyAstra_17 LadyAstra_18 LadyAstra_19 LadyAstra_20 LadyAstra_21 LadyAstra_22 LadyAstra_23 LadyAstra_24 LadyAstra_25 LadyAstra_26 LadyAstra_27 LadyAstra_28 LadyAstra_29 LadyAstra_30 LadyAstra_31 LadyAstra_32 LadyAstra_33 LadyAstra_34 LadyAstra_35 LadyAstra_36 LadyAstra_37 LadyAstra_38 LadyAstra_39 LadyAstra_40 LadyAstra_41 LadyAstra_42 LadyAstra_43 LadyAstra_44 LadyAstra_45 LadyAstra_46 LadyAstra_47 LadyAstra_48 LadyAstra_49 LadyAstra_50 LadyAstra_51 LadyAstra_52 LadyAstra_53 LadyAstra_54 LadyAstra_55 LadyAstra_56 LadyAstra_57 LadyAstra_58 LadyAstra_59 LadyAstra_60 LadyAstra_61 LadyAstra_62 LadyAstra_63 LadyAstra_64 LadyAstra_65 LadyAstra_66 LadyAstra_67 LadyAstra_68 LadyAstra_69 — tRNA LadyAstra_70 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
