| Detailed Information for Phage Lorelei |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Emily Smith |
| Year Found | 2015 |
| Location Found | Beaumont, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.099116 N, 94.214458 W Map |
| Discovery Notes | I collected the soil on September 6, 2015. It was 92 degrees F outside and very humid. The soil I collected was wet and rich with organic material, it contained a small amount of clay. |
| Naming Notes | The name of this phage came from a German myth about a woman who threw herself into a river after being heartbroken. She was then transformed into a water spirit that lured men to their death. I chose to use the name because my phage was very distinctive and had a tendency to completely lyse plates so I decided the name was appropriate. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3632 |
| Genome length (bp) | 50558 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGCCAGTCAT |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are very large, 2-3 mm on average. They are very round and clear, with crisp edges. They generally clustered and created sizeable lysed areas in the bacteria. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX507343 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0 x 10^7 pfu/mL |
| Date of SEA Lysate Titering |
Nov 15, 2015 |
| Pitt Freezer Box# |
20 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
