| Detailed Information for Phage Margaret |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.46256 N, 79.9319 W Map |
| Discovery Notes | This phage was isolated from the plant Trifolium repens, which was collected by Andrea Fetters. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 6, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1102 |
| Genome length (bp) | 46950 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCAT |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Variable morphology--mostly small plaques, but plaques become larger when fewer PFU are plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH271302 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
