| Discovery Information |
| Isolation Host | Arthrobacter atrocyaneus NRRL B-2883 |
| Found By | Cristal Santaolaya |
| Year Found | 2023 |
| Location Found | Shadow Hills, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.2728 N, 118.33717 W Map |
| Discovery Notes | Moist and slightly saturated soil that contained some small ricks, shrubbery, and grass. Near a small stream that flowed down from a mountain. The surroundings were rocky and had lots of vegetation. Collection site was near a highway and even flowed beneath a freeway bridge where the ground was flat.
AL Quarter and Lab section (ie. F19-1A): S23-1A
Instructor: Dr. Tejas Bouklas
Teaching Assistant: Dominic Garza
Undergraduate Assistant(s): Samantha Padilla, Trinity Vu
Team #1 Name: Phantom Phages
Team #1 Members: Elisha Anne Santos, Emma Yang, Kaley Qin, Cristal Santaolaya |
| Naming Notes | The plaques formed by the phage are clear, circular, and with sharp edges. When there are multiple PFU on a bacterial lawn, everything looks like a piece of swiss cheese. Thus, we named her MissSwiss. Also, all of the members on this team really like cheese. :) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2756 |
| Genome length (bp) | 42615 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGAGGCAT |
| End Determination Notes | at position 15912 bp 50% of bases contain an A to C at substitution |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology (e.g., halos, clear or turbid): Circular and clear with sharp edged margins
Average size (and size range) diameter in mm: Range is between 1-2 mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978848 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
