| Detailed Information for Phage Msay19 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Robert Sayce |
| Year Found | 2017 |
| Location Found | PITTSBURGH, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | -11°C |
| GPS Coordinates | 40.444444 N, 79.954722 W Map |
| Discovery Notes | This phage was discovered in front of the cathedral of learning on 01/08/2017, the temperature at time of collection was 12 degrees Fahrenheit. This phage came from a sample collected by Kristin Link. |
| Naming Notes | This phage was named using the first letter of someone I miss dearly and the first letters of my last name, and 19 being my favorite number. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1420 |
| Genome length (bp) | 77513 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 59.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | This forms a small relatively clear plaque with cloudy plaques found on alternate hosts |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK433261 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4x10^10pfu/mL |
| Date of SEA Lysate Titering |
Mar 1, 2017 |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
A9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
