| Detailed Information for Phage Paolo |
| Discovery Information |
| Isolation Host | Streptomyces baarnensis NRRL B-2842 |
| Found By | Daniel Schonfeld |
| Year Found | 2023 |
| Location Found | Frisco, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.21674 N, 96.86729 W Map |
| Discovery Notes | My soil sample was collected on January 22, 2023. The temperature of the location was at 39 degrees F and the humidity level was at 76%. The 2 inches worth of soil that I used for the sample was dry and came out in chunks. |
| Naming Notes | I chose the name “Paolo” because it’s the name of my favorite bass guitar player. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2486 |
| Genome length (bp) | 51580 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCTACGTCTT |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques had a slight halo look to them and measured to and average diameter of .6mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978823 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^9 |
| Pitt Freezer Box# |
174 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
