Streptomyces phage Paolo
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Detailed Information for Phage Paolo
Discovery Information
Isolation HostStreptomyces baarnensis NRRL B-2842
Found ByDaniel Schonfeld
Year Found2023
Location FoundFrisco, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.21674 N, 96.86729 W Map
Discovery NotesMy soil sample was collected on January 22, 2023. The temperature of the location was at 39 degrees F and the humidity level was at 76%. The 2 inches worth of soil that I used for the sample was dry and came out in chunks.
Naming NotesI chose the name “Paolo” because it’s the name of my favorite bass guitar player.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2486
Genome length (bp)51580
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCTACGTCTT
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Other Cluster Members

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Aaronocolus (BD1)

Asten (BD1)

BarryBee (BD1)

BeardedLady (BD1)

Bovely (BD1)

Brataylor (BD1)

BryanRecycles (BD1)

Caliburn (BD1)

Celeste (BD1)

Chucky (BD1)

Danzina (BD1)

Dattran (BD1)

Dwayne (BD1)

Eddasa (BD1)

Ejemplo (BD1)

Emaanora (BD1)

EnochSoames (BD1)

Esperer (BD1)

Goby (BD1)

Godpower (BD1)

Hippo (BD1)

Hydra (BD1)

Indigo (BD1)

Izzy (BD1)

Jash (BD1)

Katalie (BD1)

Lannister (BD1)

Legacy (BD1)

Leviticus (BD1)

Lika (BD1)

Lorelei (BD1)

Maneekul (BD1)

Nabi (BD1)

Nanodon (BD1)

Nerdos (BD1)

Oliynyk (BD1)

Ozzie (BD1)

OzzyJ (BD1)

Phettuccine (BD1)

Rana (BD1)

RedBear (BD1)

Rusticus (BD1)

SarahRose (BD1)

Snorlax (BD1)

South40 (BD1)

Sujidade (BD1)

SunsetPointe (BD1)

TagePhighter (BD1)

Toma (BD1)

Triste (BD1)

TuanPN (BD1)

Unstoppable (BD1)

Werner (BD1)

Whatever (BD1)

Yasdnil (BD1)

Zemlya (BD1)

Alvy (BD2)

Amethyst (BD2)

Andris (BD2)

Animus (BD2)

BartholomewSD (BD2)

Bowden (BD2)

Caelum (BD2)

Daudau (BD2)

Diane (BD2)

ELB20 (BD2)

GirlDinner (BD2)

Haizum (BD2)

Hank144 (BD2)

Issmi (BD2)

Janus (BD2)

Jevington (BD2)

Loofah (BD2)

Marav (BD2)

Nishikigoi (BD2)

Omar (BD2)

Pablito (BD2)

Paedore (BD2)

Paolo (BD2)

Puginator (BD2)

R4 (BD2)

SqueakyClean (BD2)

Superstar (BD2)

Tefunt (BD2)

Thestral (BD2)

TinaBelcher (BD2)

Triumph (BD2)

TrvxScott (BD2)

Zainub (BD2)

Alsaber (BD3)

Amela (BD3)

Celery (BD3)

Conan (BD3)

Dexers (BD3)

ElGato (BD3)

Kaine (BD3)

Pavo (BD3)

phiCAM (BD3)

Provolone (BD3)

Saftant (BD3)

Speedwell (BD3)

Vanseggelen (BD3)

Verabelle (BD3)

Verse (BD3)

Yosif (BD3)

BroPlease (BD4)

GreenWeasel (BD4)

phiHau3 (BD4)

StrepC (BD5)

Celia (BD6)

Itza (BD6)

Urza (BD6)

VieEnRose (BD6)

Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesThe plaques had a slight halo look to them and measured to and average diameter of .6mm.
MorphotypeSiphoviridae
Number of Genes83
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Paolo_1

Paolo_2
(helix-turn-helix DNA binding domain)

Paolo_3
(HNH endonuclease)

Paolo_4 — tRNA
(tRNA-Trp(cca))

Paolo_5

Paolo_6
(thioredoxin)

Paolo_7

Paolo_8
(terminase large subunit)

Paolo_9
(portal protein)

Paolo_10

Paolo_11
(scaffolding protein)

Paolo_12
(major capsid protein)

Paolo_13
(head-to-tail adaptor)

Paolo_14
(head-to-tail stopper)

Paolo_15

Paolo_16
(tail terminator)

Paolo_17
(major tail protein)

Paolo_18
(tail assembly chaperone)

Paolo_19
(tail assembly chaperone)

Paolo_20
(tape measure protein)

Paolo_21
(minor tail protein)

Paolo_22
(minor tail protein)

Paolo_23

Paolo_24
(minor tail protein)

Paolo_25

Paolo_26
(minor tail protein)

Paolo_27

Paolo_28
(endolysin)

Paolo_29

Paolo_30
(DNA binding protein)

Paolo_31
(immunity repressor)

Paolo_32

Paolo_33
(exonuclease)

Paolo_34

Paolo_35

Paolo_36

Paolo_37
(deoxycytidylate deaminase)

Paolo_38
(DnaB-like dsDNA helicase)

Paolo_39
(Holliday junction resolvase)

Paolo_40

Paolo_41
(DNA primase)

Paolo_42
(DNA primase)

Paolo_43

Paolo_44

Paolo_45
(helix-turn-helix DNA binding domain)

Paolo_46
(MRE11 double-strand break endo/exonuclease)

Paolo_47

Paolo_48
(DNA binding protein)

Paolo_49

Paolo_50
(ribonucleotide reductase)

Paolo_51

Paolo_52

Paolo_53
(DNA binding protein)

Paolo_54
(serine integrase)

Paolo_55

Paolo_56
(ThyX-like thymidylate synthase)

Paolo_57

Paolo_58

Paolo_59

Paolo_60

Paolo_61

Paolo_62

Paolo_63

Paolo_64

Paolo_65

Paolo_66
(deoxynucleoside monophosphate kinase)

Paolo_67

Paolo_68

Paolo_69

Paolo_70

Paolo_71

Paolo_72

Paolo_73

Paolo_74

Paolo_75

Paolo_76

Paolo_77

Paolo_78

Paolo_79
(acetyltransferase)

Paolo_80

Paolo_81

Paolo_82

Paolo_83

Paolo_84

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978823
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^9
Pitt Freezer Box# 174
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload