| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Hilory Foster |
| Year Found | 2019 |
| Location Found | Valencia, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 34.443624 N, 118.609545 W Map |
| Discovery Notes | The sample was collected by Hilory Foster in Los Angeles, CA. Arthrobacter phage Powerpuff was isolated from soil by enrichment plating at 25°C and was characterized and annotated by Pauline E. Aghamalian, Anya S. Alag, Erika M. Capehart, Hilory Foster, Daisy Hernandez-Casas, Sophia C. Wang, Heather A. Angel, Abbas Hakim, and Derek Q. Lam.
Instructor: Amanda Freise
Teaching Assistants: Ryan Ngo, Andrew Kapinos
Undergraduate Assistants: Yvette Lakkis, Andrew Lee |
| Naming Notes | It was named after the Powerpuff girls because they were powerful and this phage is also powerful by being able to lyse through a whole plate. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 705 |
| Genome length (bp) | 44651 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Different conditions have produced either bullseye or completely turbid plaques and, in a few cases, clear plaques have been observed. The switch from bullseye plaques to turbid/clear plaques indicate a switch in life cycle which is indicative of the temperate life cycle.
Serine integrase was identified during annotation. |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | turbid and bullseye
Average diameter: 1mm |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN703413 |
| Refseq Number | None yet |
| Published in Paper | Kapinos et al 2022, PLOS ONE: Novel Cluster AZ Arthrobacter phages Powerpuff, Lego, and YesChef exhibit close functional relationships with Microbacterium phages |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
116 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
