Streptomyces phage Puginator
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Detailed Information for Phage Puginator
Discovery Information
Isolation HostStreptomyces baarnensis NRRL B-2842
Found ByDalya Kinsizer
Year Found2023
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.17401 N, 97.09913 W Map
Discovery NotesDamp soil was collected on 1/22/23. It was 12 degrees Celsius, 34% humidity, and 29.99 Hg air pressure.
Naming NotesNamed in honor of my neighbors' baby pug's favorite spot to dig.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2413
Genome length (bp)51731
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Other Cluster Members

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Aaronocolus (BD1)

Asten (BD1)

BarryBee (BD1)

BeardedLady (BD1)

Bovely (BD1)

Brataylor (BD1)

BryanRecycles (BD1)

Caliburn (BD1)

Celeste (BD1)

Chucky (BD1)

Danzina (BD1)

Dattran (BD1)

Dwayne (BD1)

Eddasa (BD1)

Ejemplo (BD1)

Emaanora (BD1)

EnochSoames (BD1)

Esperer (BD1)

Goby (BD1)

Godpower (BD1)

Hippo (BD1)

Hydra (BD1)

Indigo (BD1)

Izzy (BD1)

Jash (BD1)

Katalie (BD1)

Lannister (BD1)

Legacy (BD1)

Leviticus (BD1)

Lika (BD1)

Lorelei (BD1)

Maneekul (BD1)

Nabi (BD1)

Nanodon (BD1)

Nerdos (BD1)

Oliynyk (BD1)

Ozzie (BD1)

OzzyJ (BD1)

Phettuccine (BD1)

Rana (BD1)

RedBear (BD1)

Rusticus (BD1)

SarahRose (BD1)

Snorlax (BD1)

South40 (BD1)

Sujidade (BD1)

SunsetPointe (BD1)

TagePhighter (BD1)

Toma (BD1)

Triste (BD1)

TuanPN (BD1)

Unstoppable (BD1)

Werner (BD1)

Whatever (BD1)

Yasdnil (BD1)

Zemlya (BD1)

Alvy (BD2)

Amethyst (BD2)

Andris (BD2)

Animus (BD2)

BartholomewSD (BD2)

Bowden (BD2)

Caelum (BD2)

Daudau (BD2)

Diane (BD2)

ELB20 (BD2)

GirlDinner (BD2)

Haizum (BD2)

Hank144 (BD2)

Issmi (BD2)

Janus (BD2)

Jevington (BD2)

Loofah (BD2)

Marav (BD2)

Nishikigoi (BD2)

Omar (BD2)

Pablito (BD2)

Paedore (BD2)

Paolo (BD2)

Puginator (BD2)

R4 (BD2)

SqueakyClean (BD2)

Superstar (BD2)

Tefunt (BD2)

Thestral (BD2)

TinaBelcher (BD2)

Triumph (BD2)

TrvxScott (BD2)

Zainub (BD2)

Alsaber (BD3)

Amela (BD3)

Celery (BD3)

Conan (BD3)

Dexers (BD3)

ElGato (BD3)

Kaine (BD3)

Pavo (BD3)

phiCAM (BD3)

Provolone (BD3)

Saftant (BD3)

Speedwell (BD3)

Vanseggelen (BD3)

Verabelle (BD3)

Verse (BD3)

Yosif (BD3)

BroPlease (BD4)

GreenWeasel (BD4)

phiHau3 (BD4)

StrepC (BD5)

Celia (BD6)

Itza (BD6)

Urza (BD6)

VieEnRose (BD6)

Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesClear, circular, dense, and ranging in size. The average diameter is 0.5 mm.
MorphotypeSiphoviridae
Number of Genes83
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Puginator_1

Puginator_2
(DNA binding protein)

Puginator_3
(HNH endonuclease)

Puginator_4 — tRNA
(tRNA-Phe(gaa))

Puginator_5

Puginator_6
(thioredoxin)

Puginator_7

Puginator_8
(terminase large subunit)

Puginator_9
(portal protein)

Puginator_10

Puginator_11
(scaffolding protein)

Puginator_12
(major capsid protein)

Puginator_13
(head-to-tail adaptor)

Puginator_14
(head-to-tail stopper)

Puginator_15

Puginator_16
(tail terminator)

Puginator_17
(major tail protein)

Puginator_18
(tail assembly chaperone)

Puginator_19
(tail assembly chaperone)

Puginator_20
(tape measure protein)

Puginator_21
(minor tail protein)

Puginator_22
(minor tail protein)

Puginator_23

Puginator_24
(minor tail protein)

Puginator_25

Puginator_26
(minor tail protein)

Puginator_27

Puginator_28
(endolysin)

Puginator_29

Puginator_30
(DNA binding protein)

Puginator_31
(immunity repressor)

Puginator_32

Puginator_33
(exonuclease)

Puginator_34

Puginator_35

Puginator_36

Puginator_37
(deoxycytidylate deaminase)

Puginator_38
(DnaB-like dsDNA helicase)

Puginator_39
(Holliday junction resolvase)

Puginator_40

Puginator_41
(DNA primase)

Puginator_42
(DNA primase)

Puginator_43

Puginator_44

Puginator_45
(helix-turn-helix DNA binding domain)

Puginator_46
(MRE11 double-strand break endo/exonuclease)

Puginator_47

Puginator_48
(DNA binding protein)

Puginator_49

Puginator_50
(ribonucleotide reductase)

Puginator_51

Puginator_52

Puginator_53
(DNA binding protein)

Puginator_54
(serine integrase)

Puginator_55

Puginator_56
(ThyX-like thymidylate synthase)

Puginator_57

Puginator_58

Puginator_59

Puginator_60

Puginator_61

Puginator_62

Puginator_63

Puginator_64

Puginator_65

Puginator_66
(deoxynucleoside monophosphate kinase)

Puginator_67

Puginator_68

Puginator_69

Puginator_70

Puginator_71

Puginator_72

Puginator_73

Puginator_74

Puginator_75

Puginator_76

Puginator_77

Puginator_78

Puginator_79
(acetyltransferase)

Puginator_80

Puginator_81

Puginator_82

Puginator_83

Puginator_84

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978857
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6x10^8
Pitt Freezer Box# 174
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload