| Detailed Information for Phage Sabourin |
| Discovery Information |
| Isolation Host | Curtobacterium flaccumfaciens NRRL B-59340 |
| Found By | Isabel Dauphinais and Michelle Dacri |
| Year Found | 2024 |
| Location Found | Orono, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 20°C |
| GPS Coordinates | 44.541 N, 68.405 W Map |
| Discovery Notes | The soil was acquired at MLK Plaza at the University of Maine under a tree on September 4, 2024. The temperature on that night was 68 degrees Fahrenheit. |
| Naming Notes | This phage was named after Isabel's step-great-grandfather. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 7, 2025 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Single-end reads |
| Read Length | 250 bp |
| Approximate Shotgun Coverage | 71886 |
| Genome length (bp) | 40042 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAACGGCAT |
| GC Content | 70.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Overdue (Expected completion by 5/1/2025) |
| Plaque Notes | The plaque morphology of the phage varies from 0.5mm to 8mm in diameter. Most of the plaques are clear with a turbid ring; however, some plaques are clear with defined edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
199 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
