| Detailed Information for Phage ShakeItOph |
| Discovery Information |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Duplicates of this Phage | SimonMango |
| Found By | Mrudula Akkinepally |
| Year Found | 2023 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.067783 N, 118.450455 W Map |
| Discovery Notes | Soil from suburban environment. Environment was not sloped. Sample collected from a mini garden space on a balcony near a busy street at 5cm deep and ambient temperature of 23 deg C.
Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation.
AL Quarter and Lab section: F23-1A
Instructor: Dr. Tejas Bouklas
Teaching Assistant: Dom Garza
Undergraduate Assistant(s): Geethika Janga and Saineha Maddineni
Team #1 Name: Plaque to December
Team #1 Members: Hannah Joo, Alessia Giusti, Bella Garcia, and Mrudula Akkinepally |
| Naming Notes | Named after Taylor Swift's Shake It Off |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1357 |
| Genome length (bp) | 43672 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAACTGGCAT |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Bullseye plaque; 20mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915865 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |