| Detailed Information for Phage ShakeItOph | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Duplicates of this Phage | SimonMango |
| Found By | Mrudula Akkinepally |
| Year Found | 2023 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.067783 N, 118.450455 W Map |
| Discovery Notes | Soil from suburban environment. Environment was not sloped. Sample collected from a mini garden space on a balcony near a busy street at 5cm deep and ambient temperature of 23 deg C. Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: F23-1A Instructor: Dr. Tejas Bouklas Teaching Assistant: Dom Garza Undergraduate Assistant(s): Geethika Janga and Saineha Maddineni Team #1 Name: Plaque to December Team #1 Members: Hannah Joo, Alessia Giusti, Bella Garcia, and Mrudula Akkinepally |
| Naming Notes | Named after Taylor Swift's Shake It Off |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1357 |
| Genome length (bp) | 43672 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAACTGGCAT |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) Sooty (AZ5) SpecialK (AZ5) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Bullseye plaque; 20mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewShakeItOph_1 ShakeItOph_2 ShakeItOph_3 ShakeItOph_4 ShakeItOph_5 ShakeItOph_6 ShakeItOph_7 ShakeItOph_8 ShakeItOph_9 ShakeItOph_10 ShakeItOph_11 ShakeItOph_12 ShakeItOph_13 ShakeItOph_14 ShakeItOph_15 ShakeItOph_16 ShakeItOph_17 ShakeItOph_18 ShakeItOph_19 ShakeItOph_20 ShakeItOph_21 ShakeItOph_22 ShakeItOph_23 ShakeItOph_24 ShakeItOph_25 ShakeItOph_26 ShakeItOph_27 ShakeItOph_28 ShakeItOph_29 ShakeItOph_30 ShakeItOph_31 ShakeItOph_32 ShakeItOph_33 ShakeItOph_34 ShakeItOph_35 ShakeItOph_36 ShakeItOph_37 ShakeItOph_38 ShakeItOph_39 ShakeItOph_40 ShakeItOph_41 ShakeItOph_42 ShakeItOph_43 ShakeItOph_44 ShakeItOph_45 ShakeItOph_46 ShakeItOph_47 ShakeItOph_48 ShakeItOph_49 ShakeItOph_50 ShakeItOph_51 ShakeItOph_52 ShakeItOph_53 ShakeItOph_54 ShakeItOph_55 ShakeItOph_56 ShakeItOph_57 ShakeItOph_58 ShakeItOph_59 ShakeItOph_60 ShakeItOph_61 ShakeItOph_62 ShakeItOph_63 ShakeItOph_64 ShakeItOph_65 ShakeItOph_66 ShakeItOph_67 ShakeItOph_68 ShakeItOph_69 ShakeItOph_70 — tRNA ShakeItOph_71 ShakeItOph_72 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | E10 |
| Available Files | |
| EM Picture | Download |