| Detailed Information for Phage Superstar |
| Discovery Information |
| Isolation Host | Streptomyces avermitilis NRRL 8165 |
| Found By | Emily Wood |
| Year Found | 2023 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 20°C |
| GPS Coordinates | 45.38023 N, 75.6774 W Map |
| Discovery Notes | Superstar was discovered in soil underneath a fruit tree in the Heron Park Community Orchard in Ottawa, ON, Canada. Sample was collected from 1cm below the surface and was dry/ sandy, packed hard and cold.
Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry. The sequence assembled into one contig of 38,425bp with 97% query cover, 94.80% identity to Spernnie (BD2). The sequence was assembled into a second shorter contig of 2,607bp with 99% query cover, 97.09% identity to Spernnie (BD2). |
| Naming Notes | This phage is named after the Taylor Swift song Superstar! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 628 |
| Genome length (bp) | 50411 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCACGTCTT |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | Superstars' plaques vary slightly in medium sizes. They are not perfectly circular, have bumpy edges. Plaques are fairly clear, some early isolations exhibit the bacterial lawn growing back in the plaque from the edges, producing a turbid halo effect. |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
176 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
