Detailed Information for Phage Thestral |
Discovery Information |
Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
Found By | Megan Webb |
Year Found | 2016 |
Location Found | Denton, TX USA |
Finding Institution | University of North Texas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 33.203442 N, 97.15483 W Map |
Discovery Notes | I collected my soil sample from a pond in Denton. The soil was completed submerged in water with algae present. Animals, mainly ducks, frequent the pond. |
Naming Notes | I chose the name Thestral after the "death horses" in Harry Potter. I feel the same way for the fictional creatures as I do phages: weirded-out but interested. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Sep 7, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 304 |
Genome length (bp) | 52628 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGCCACGTCTT |
GC Content | 67.7% |
Sequencing Notes | upon assembly 2 cluster BD2's were found in the original sample, they were separated and PCR verified. Mixed phage was named Haizum
|
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BD |
Subcluster | BD2 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | My phage is not a temperate phage. |
Other Cluster Members |
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Annotating Institution | University of North Texas |
Annotation Status | In GenBank |
Morphotype | Siphoviridae |
Number of Genes | 82 |
Number of tRNAs | 1 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MH651190 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
2x10^8 pfu/mL |
Pitt Freezer Box# |
70 |
Pitt Freezer Box Grid# |
G5 |
Available Files |
Plaque Picture | Download |
GenBank File for Phamerator | Download |