| Detailed Information for Phage Thestral |
| Discovery Information |
| Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
| Found By | Megan Webb |
| Year Found | 2016 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.203442 N, 97.15483 W Map |
| Discovery Notes | I collected my soil sample from a pond in Denton. The soil was completed submerged in water with algae present. Animals, mainly ducks, frequent the pond. |
| Naming Notes | I chose the name Thestral after the "death horses" in Harry Potter. I feel the same way for the fictional creatures as I do phages: weirded-out but interested. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 7, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 304 |
| Genome length (bp) | 52628 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCACGTCTT |
| GC Content | 67.7% |
| Sequencing Notes | upon assembly 2 cluster BD2's were found in the original sample, they were separated and PCR verified. Mixed phage was named Haizum
|
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | My phage is not a temperate phage. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651190 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^8 pfu/mL |
| Pitt Freezer Box# |
70 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
