| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Edward Lee |
| Year Found | 2019 |
| Location Found | Los Angeles, CA, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.052707 N, 118.44657 W Map |
| Discovery Notes | Arthrobacter phage YesChef was isolated by Edward Lee from soil collected near trees, tall grasses, next to a playground, and municipal site, using direct plating at 30C. The phage was characterized by Team Can’t Phage Us (Edward Lee, Aaron Bernardino, Stephanie Demo).
Instructor: Amanda Freise
Teaching Assistant: Andrew Kapinos
Undergraduate Assistants: Emily Pham and Oscar Lim |
| Naming Notes | Whenever our TA told us to do something, we started replying with “yes chef” and “thank you chef” like in Hell’s Kitchen/Masterchef. We respect our TA like he is the Gordon Ramsay of virology. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2419 |
| Genome length (bp) | 43510 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: bullseye
Average size (and size range) diameter in mm: 4mm |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT024871 |
| Refseq Number | None yet |
| Published in Paper | Kapinos et al 2022, PLOS ONE: Novel Cluster AZ Arthrobacter phages Powerpuff, Lego, and YesChef exhibit close functional relationships with Microbacterium phages |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
116 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
