| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Former names | TDDu |
| Found By | Tony Du |
| Year Found | 2018 |
| Location Found | Carrollton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.001746 N, 96.864174 W Map |
| Discovery Notes | Found on 01/28/2018 at 12:00pm on a sunny day where the weather was at 21℃. The soil was collected right by an elementary school underneath a large tree. The soil itself was very crumbly and dry, with no signs of moisture at the time of collection. The color was extremely dark brown, verging on black. |
| Naming Notes | "Animus" is a Latin word that is translated to mean the soul, spirit, and the mind. I had wanted to name it after such a word because of the same things we instill into these phages upon its discovery. I had given a soul, per say, to an organism that had previously been unknown to the entire world, giving it a name to embody the very sensation many other students have when discovering their own bacteriophages. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 320 |
| Genome length (bp) | 50793 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCGTGTCTT |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Regarding lysogeny, because a spot was seen on the ten to the zero spot and no spots were seen on any of the dilution spots, this showed evidence that Animus’ phage particles overwhelmed the host bacteria, causing it to die, not because the phage infected the host. Because of this, it could mean Animus could produce a lysogen, where it would go through the lysogenic life cycle. This brings up the possibility that Animus would be classified as a temperate phage but regardless, we cannot definitively say that Animus is able to actually go through the lysogenic life cycle— further testing would need to be done. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Each of the plaques ranged anywhere between 0.5 mm and 1 mm in diameter and were all comet shaped. |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234192 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
75 |
| Pitt Freezer Box Grid# |
A6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
