Mycobacterium phage Atcoo
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| Detailed Information for Phage Atcoo | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mitali Athavale, Taylor Cook |
| Year Found | 2017 |
| Location Found | Parker, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.6095 N, 104.8819 W Map |
| Discovery Notes | The phage was isolated from potting soil in a tomato plate garden. The temperature was 33 degrees Celsius at noon. |
| Naming Notes | The name is a combination of our last names, Cook and Athavale. It also sounds cute and like a sneeze! |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 1, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 174 |
| Genome length (bp) | 49075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCTGCCGCCCCGG |
| GC Content | 67.0% |
| Sequencing Notes | This phage was sequenced as part of a mixed DOGEMS sample. The sequence of this genome was confirmed to belong to this phage by PCR. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | The diameter of our phages plaques was 0.3 cm. The plaques are clear with a definitive outline. The time it was incubated for was 48hrs and the temperature was 37 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN585977 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 61 |
| Pitt Freezer Box Grid# | E8 |
| Available Files | |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |