Mycobacterium phage Nazo
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Detailed Information for Phage Nazo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNaazneen Adam
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.917388 S, 30.899609 E Map
Discovery NotesThis phage was isolated from my mothers coriander garden.The soil her was moist but not very wet. the soil was taken from the top of the garden no more than 2cm below the surface. This was one first soil samples that had been enriched but was only plated a week after the soil had been incubated.
Naming NotesThe phage was named after myself, as it had taken a lot of hard work to actually find it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1767
Genome length (bp)48870
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCCCCCCG
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques were interesting and cool looking as they could almost not been seen. They were very opaque with a tiny clearing in the center on more that 1mm in diameter, the plaques itself were about 5mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX641262
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# E12
Available Files
Final DNAMaster FileDownload