Mycobacterium phage StressBall
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Detailed Information for Phage StressBall
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCameron Hartzler and Raul Martinez
Year Found2018
Location FoundLongview, TX USA
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.464719 N, 94.727251 W Map
Discovery NotesWe collected our phage at 9:17 am on 9/27/18. The weather was 62 degrees Fahrenheit and cloudy. The soil was a dark brown color and was originally covered with compositing leaves. We dug in the flower bed behind our science building right next to a patch of monkey grass. We made sure that the hole was about 2 inches deep.
Naming NotesFor the first 7 weeks of our adventure, we had no progress whatsoever. Even after we found our phage, one of us (Cameron) still experienced more-than-normal stress levels. He used a stress ball to try and calm his nerves, so we decided to name our phage just that.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage591
Genome length (bp)47915
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCTGCCGCCCCGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusOverdue (Expected completion by 6/14/2019)
Plaque NotesThe average plaque diameter after 5 days of incubation at 37 degrees Celsius is 2.2 mm. The plaques are almost circular, but they have a jagged edge at one end of them.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
EM PictureDownload