Mycobacterium phage StressBall
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Detailed Information for Phage StressBall
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCameron Hartzler and Raul Martinez
Year Found2018
Location FoundLongview, TX USA
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.464719 N, 94.727251 W Map
Discovery NotesWe collected our phage at 9:17 am on 9/27/18. The weather was 62 degrees Fahrenheit and cloudy. The soil was a dark brown color and was originally covered with compositing leaves. We dug in the flower bed behind our science building right next to a patch of monkey grass. We made sure that the hole was about 2 inches deep.
Naming NotesFor the first 7 weeks of our adventure, we had no progress whatsoever. Even after we found our phage, one of us (Cameron) still experienced more-than-normal stress levels. He used a stress ball to try and calm his nerves, so we decided to name our phage just that.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage591
Genome length (bp)47915
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCTGCCGCCCCGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Plaque NotesThe average plaque diameter after 5 days of incubation at 37 degrees Celsius is 2.2 mm. The plaques are almost circular, but they have a jagged edge at one end of them.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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StressBall_1
(terminase small subunit)

StressBall_2
(terminase large subunit)

StressBall_3
(portal protein)

StressBall_4
(capsid maturation protease)

StressBall_5
(scaffolding protein)

StressBall_6
(major capsid protein)

StressBall_7

StressBall_8
(head-to-tail adaptor)

StressBall_9
(head-to-tail stopper)

StressBall_10

StressBall_11

StressBall_12

StressBall_13
(tail terminator)

StressBall_14
(major tail protein)

StressBall_15
(tail assembly chaperone)

StressBall_16
(tail assembly chaperone)

StressBall_17
(tape measure protein)

StressBall_18
(minor tail protein)

StressBall_19
(minor tail protein)

StressBall_20
(minor tail protein)

StressBall_21
(minor tail protein)

StressBall_22
(minor tail protein)

StressBall_23

StressBall_24

StressBall_25

StressBall_26
(lysin A)

StressBall_27
(lysin B)

StressBall_28

StressBall_29

StressBall_30
(tyrosine integrase)

StressBall_31
(immunity repressor)

StressBall_32
(excise)

StressBall_33

StressBall_34
(antirepressor)

StressBall_35

StressBall_36

StressBall_37

StressBall_38

StressBall_39

StressBall_40

StressBall_41

StressBall_42

StressBall_43

StressBall_44

StressBall_45
(RecE-like exonuclease)

StressBall_46
(RecT-like ssDNA binding protein)

StressBall_47

StressBall_48

StressBall_49
(FtsK-like DNA translocase)

StressBall_50
(RusA-like resolvase)

StressBall_51

StressBall_52

StressBall_53
(DNA methylase)

StressBall_54

StressBall_55
(DNA methylase)

StressBall_56

StressBall_57
(helix-turn-helix DNA binding domain protein)

StressBall_58

StressBall_59

StressBall_60

StressBall_61

StressBall_62
(helix-turn-helix DNA binding domain protein)

StressBall_63
(DnaC-like helicase loader)

StressBall_64

StressBall_65
(helix-turn-helix DNA binding protein)

StressBall_66

StressBall_67

StressBall_68

StressBall_69

StressBall_70

StressBall_71

StressBall_72

StressBall_73

StressBall_74
(ribbon-helix-helix DNA binding domain protein)

StressBall_75

StressBall_76

StressBall_77

StressBall_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN908683
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload