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Ixel (EA11) Nebulous (EA5) Gandalph (F1) Coco12 (F1) Rebel (N)
Mycobacterium phage GreaseLightnin
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Detailed Information for Phage GreaseLightnin
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRichard Stauffer and Nic Bussian
Year Found2013
Location FoundKenosha, WI USA
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.6194 N, 87.8212 W Map
Discovery NotesThe soil sample was taken from underneath a tree next to a campus building, underneath some leaves and wood chips.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 22, 2015
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage8284
Genome length (bp)48424
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionCarthage College
Annotation StatusIn GenBank
Plaque NotesThe plaque morphology, after incubated for 48 hours, consists of each plaque having a diameter of approximately 1 mm, each plaque is clear, and there was no variation in this plaque morphology.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

GreaseLightnin_1
(terminase small subunit)

GreaseLightnin_2
(terminase large subunit)

GreaseLightnin_3
(portal protein)

GreaseLightnin_4
(capsid maturation protease)

GreaseLightnin_5
(scaffolding protein)

GreaseLightnin_6
(major capsid protein)

GreaseLightnin_7

GreaseLightnin_8
(head-to-tail adaptor)

GreaseLightnin_9
(head-to-tail stopper)

GreaseLightnin_10

GreaseLightnin_11

GreaseLightnin_12

GreaseLightnin_13
(tail terminator)

GreaseLightnin_14
(major tail protein)

GreaseLightnin_15
(tail assembly chaperone)

GreaseLightnin_16
(tail assembly chaperone)

GreaseLightnin_17
(tapemeasure protein)

GreaseLightnin_18
(minor tail protein)

GreaseLightnin_19
(minor tail protein)

GreaseLightnin_20
(minor tail protein)

GreaseLightnin_21
(minor tail protein)

GreaseLightnin_22
(minor tail protein)

GreaseLightnin_23

GreaseLightnin_24

GreaseLightnin_25

GreaseLightnin_26
(lysin A)

GreaseLightnin_27
(lysin B)

GreaseLightnin_28
(holin)

GreaseLightnin_29

GreaseLightnin_30
(integrase)

GreaseLightnin_31
(immunity repressor)

GreaseLightnin_32

GreaseLightnin_33

GreaseLightnin_34
(antirepressor)

GreaseLightnin_35

GreaseLightnin_36

GreaseLightnin_37

GreaseLightnin_38

GreaseLightnin_39

GreaseLightnin_40

GreaseLightnin_41

GreaseLightnin_42

GreaseLightnin_43

GreaseLightnin_44

GreaseLightnin_45

GreaseLightnin_46
(RecE-like exonuclease)

GreaseLightnin_47
(RecT-like ssDNA binding protein)

GreaseLightnin_48

GreaseLightnin_49

GreaseLightnin_50
(FtsK-like DNA translocase)

GreaseLightnin_51
(RusA-like resolvase)

GreaseLightnin_52

GreaseLightnin_53

GreaseLightnin_54

GreaseLightnin_55

GreaseLightnin_56

GreaseLightnin_57

GreaseLightnin_58
(band-7 like protein)

GreaseLightnin_59
(DNA methylase)

GreaseLightnin_60

GreaseLightnin_61
(DNA methylase)

GreaseLightnin_62
(DNA methylase)

GreaseLightnin_63
(helix-turn-helix DNA-binding protein)

GreaseLightnin_64

GreaseLightnin_65

GreaseLightnin_66

GreaseLightnin_67
(helix-turn-helix DNA-binding domain protein)

GreaseLightnin_68

GreaseLightnin_69

GreaseLightnin_70
(MerR-like HTH DNA binding protein)

GreaseLightnin_71

GreaseLightnin_72

GreaseLightnin_73

GreaseLightnin_74

GreaseLightnin_75
(ribbon-helix-helix DNA binding domain protein)

GreaseLightnin_76

GreaseLightnin_77

GreaseLightnin_78

GreaseLightnin_79

GreaseLightnin_80
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494122
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6x10^9
Pitt Freezer Box# 4
Pitt Freezer Box Grid# H2
Available Files
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload