Mycobacterium phage Phayonce
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Detailed Information for Phage Phayonce
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrooke Kihle
Year Found2014
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.444512 N, 79.954304 W Map
Discovery NotesThe soil my phage was collected from was very moist, cool and dark brown. It had rained the day before collected so the sample was a little damp.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage285
Genome length (bp)49203
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCCCCCGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP5
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notessmall virulent plaques, uniform in morphology
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Phayonce_1
(terminase small subunit)

Phayonce_2
(terminase large subunit)

Phayonce_3
(portal protein)

Phayonce_4
(capsid maturation protease)

Phayonce_5
(scaffolding protein)

Phayonce_6
(major capsid protein)

Phayonce_7

Phayonce_8
(head-to-tail adaptor)

Phayonce_9
(head-to-tail stopper)

Phayonce_10

Phayonce_11

Phayonce_12
(tail terminator)

Phayonce_13
(major tail protein)

Phayonce_14
(tail assembly chaperone)

Phayonce_15
(tail assembly chaperone)

Phayonce_16
(tape measure protein)

Phayonce_17
(minor tail protein)

Phayonce_18
(minor tail protein)

Phayonce_19
(minor tail protein)

Phayonce_20
(minor tail protein)

Phayonce_21

Phayonce_22

Phayonce_23

Phayonce_24

Phayonce_25

Phayonce_26
(lysin A)

Phayonce_27
(lysin B)

Phayonce_28

Phayonce_29

Phayonce_30

Phayonce_31

Phayonce_32
(tyrosine integrase)

Phayonce_33
(immunity repressor)

Phayonce_34

Phayonce_35

Phayonce_36
(antirepressor)

Phayonce_37

Phayonce_38

Phayonce_39

Phayonce_40

Phayonce_41

Phayonce_42

Phayonce_43
(WhiB family transcription factor)

Phayonce_44

Phayonce_45

Phayonce_46
(RecB-like exonuclease/helicase)

Phayonce_47
(RecT-like ssDNA binding protein)

Phayonce_48

Phayonce_49

Phayonce_50

Phayonce_51

Phayonce_52

Phayonce_53

Phayonce_54
(DNA methylase)

Phayonce_55

Phayonce_56

Phayonce_57

Phayonce_58

Phayonce_59

Phayonce_60

Phayonce_61

Phayonce_62

Phayonce_63

Phayonce_64

Phayonce_65
(HNH endonuclease)

Phayonce_66
(RepA-like replication initiator)

Phayonce_67

Phayonce_68

Phayonce_69
(helix-turn-helix DNA binding protein)

Phayonce_70

Phayonce_71

Phayonce_72

Phayonce_73

Phayonce_74

Phayonce_75

Phayonce_76

Phayonce_77
(adenylate kinase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKR080195
Refseq NumberNone yet
Published in PaperPope WH et al 2015, Genome Announcements: Genome sequence of Mycobacteriophage Mindy
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload