Mycobacterium phage Zilizebeth
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Detailed Information for Phage Zilizebeth
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNzinga Hendricks
Year Found2017
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.6259 N, 75.3705 W Map
Discovery NotesThe soil sample that my phage was extracted from was soil found from behind Drinker House, located on 64 Quad Drive on Lehigh University's campus. The soil was collected January 23rd, 2017, and that particular day it was raining so the soil was damp.
Naming NotesThe name for the phage is a mash-up of the second and third letter of my first name (Zi-Nzinga), with lizebeth being a modified spelling of my middle name (which is Elizabeth).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 26, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage672
Genome length (bp)48056
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.3%
Sequencing NotesThis sample was part of LEHIGH 2017 DOGEMS. Phage was PCR verified.
At position 20346 bp 25% of reads contain an A instead of consensus G.
At position 28321 bp 42% of reads contain a C instead of consensus A.
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesThe plaque morphology of Zilizebeth is the following:

The plaque clearings are very clear (no traces of turbid clearings or rings). This indicates that Zilizbeth follows a lytic cycle. The phage clearings are mixed between small phage clearings (ranging between 0.5-1.0 mm) and large clearings (ranging between 1.5-2.5 mm). The plaques form after 24 hours.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Zilizebeth_1
(terminase small subunit)

Zilizebeth_2
(terminase large subunit)

Zilizebeth_3
(portal protein)

Zilizebeth_4
(capsid maturation protease)

Zilizebeth_5
(scaffolding protein)

Zilizebeth_6
(major capsid protein)

Zilizebeth_7

Zilizebeth_8
(head-to-tail adaptor)

Zilizebeth_9
(head-to-tail stopper)

Zilizebeth_10

Zilizebeth_11

Zilizebeth_12

Zilizebeth_13
(tail terminator)

Zilizebeth_14
(major tail protein)

Zilizebeth_15
(tail assembly chaperone)

Zilizebeth_16
(tail assembly chaperone)

Zilizebeth_17
(tape measure protein)

Zilizebeth_18
(minor tail protein)

Zilizebeth_19
(minor tail protein)

Zilizebeth_20
(minor tail protein)

Zilizebeth_21
(minor tail protein)

Zilizebeth_22

Zilizebeth_23

Zilizebeth_24

Zilizebeth_25

Zilizebeth_26
(lysin A)

Zilizebeth_27
(lysin B)

Zilizebeth_28
(holin)

Zilizebeth_29

Zilizebeth_30
(integrase)

Zilizebeth_31
(immunity repressor)

Zilizebeth_32
(helix-turn-helix DNA binding domain protein)

Zilizebeth_33

Zilizebeth_34
(antirepressor)

Zilizebeth_35

Zilizebeth_36

Zilizebeth_37

Zilizebeth_38

Zilizebeth_39

Zilizebeth_40

Zilizebeth_41

Zilizebeth_42

Zilizebeth_43

Zilizebeth_44

Zilizebeth_45

Zilizebeth_46
(RecE-like exonuclease)

Zilizebeth_47
(RecT-like ssDNA binding protein)

Zilizebeth_48

Zilizebeth_49
(FtsK-like DNA translocase)

Zilizebeth_50
(RusA-like resolvase)

Zilizebeth_51

Zilizebeth_52

Zilizebeth_53

Zilizebeth_54

Zilizebeth_55

Zilizebeth_56
(helix-turn-helix DNA binding protein)

Zilizebeth_57

Zilizebeth_58

Zilizebeth_59

Zilizebeth_60

Zilizebeth_61
(helix-turn-helix DNA binding domain protein)

Zilizebeth_62

Zilizebeth_63
(DNA methylase)

Zilizebeth_64

Zilizebeth_65

Zilizebeth_66
(DNA methylase)

Zilizebeth_67

Zilizebeth_68
(helix-turn-helix DNA binding domain protein)

Zilizebeth_69

Zilizebeth_70

Zilizebeth_71
(helix-turn-helix DNA binding domain protein)

Zilizebeth_72

Zilizebeth_73

Zilizebeth_74

Zilizebeth_75

Zilizebeth_76

Zilizebeth_77

Zilizebeth_78

Zilizebeth_79
(ribbon-helix-helix DNA binding domain)

Zilizebeth_80

Zilizebeth_81

Zilizebeth_82

Zilizebeth_83
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524508
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 51
Pitt Freezer Box Grid# D9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload