Mycobacterium phage Bogie
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Detailed Information for Phage Bogie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSpook
Found ByReid Rogers
Year Found2015
Location FoundCamden, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates33.470016 N, 92.873349 W Map
Discovery NotesDepth: 1.27 cm

Moist soil

Temp: 33.8889 Celsius

Location: Latitude: 33.4700165
Longitude: -92.87334859
Sea Level: 67 m

24 August 2015 - 30 August 2015

Max- 33.8889 degrees Celsius
Mean- 23.8889 degrees Celsius
Min- 13.8889 degrees Celsius

Rained 1.1938 cm on 24 August 2015
25 August 2015 - 30 August 2015 no precipitation occured.

Soil Sample Collection Information:
. On a North facing slope
. 20.32 cm above a pool of stagnant water with film on surface
. Flowing creek 243.84 cm from sample location
. Collected under green moss with red buds that released a fine red powder
. Collected below an oak tree and holly tree
. Just above sample site a decomposing oak tree with fungus is present
. No human traffic in proximity of sample site
. Deer and raccoon traffic around sample site
. Asphalt road approximately 32 meters from collection site

Naming NotesNamed after the term ghost in phages. i.e(synonym for ghost)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2988
Genome length (bp)48639
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Lysogeny NotesFurther experiments were done with our phage in order to test its lysogeny. A serial dilution of our HTL was used for a spot test in order to determine which dilution formed the best mesa. The undiluted HTL formed the best mesa. We grew this M. smegmatis (Bogie) on a clean TA plate and tested it on a bacterial lawn for halo formation. A halo formed and the lysogen grew. Therefore, Bogie is a great lysogen candidate.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall circular plaques with a large halo on the exterior. Hazy center and cloudy edges. Temperate phage resemblance.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF133446
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.9 x 10^9
Date of SEA Lysate Titering Oct 15, 2015
Pitt Freezer Box# 22
Pitt Freezer Box Grid# E6
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload